We narrowed to 386 results for: FLU;

1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

2. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...secondary antibodies, allowing us to only image autofluorescence i.e., background noise. We then compare the...

3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...expressing these constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression...

4. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...the PSAM acts as a gating mechanism to control ion flux. The system allows you to directly manipulate ion...

5. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ...to edit The sequence you choose to install can influence the success of your prime edit. If possible, consider...

6. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...

7. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...21238511. 6. Jeong, Jae-Woong, et al. Wireless Optofluidic Systems for Programmable In Vivo Pharmacology...

8. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

   Type

   Blog Post

   Published

   Jan. 21, 2015, 1:30 p.m.

   ... Dale Carnegie’s book “How To Win Friends and Influence People.” Thank you to our guest blogger!  Dora...

9. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ...visualize proteins in vivo by tagging them with fluorescent proteins to study localization or purify proteins...

10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ...ePRIDICT can help you understand how chromatin might influence editing results at your target. Figure...

11. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...that the amino acids neighboring the Myc epitope influence 9E10’s affinity for the epitope, a phenomenon ...

12. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ... the central nervous system via cerebral spinal fluid. The injection site is the space in and around the...

13. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...characterized them by ELISA, homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After...

14. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...protein coding genes in the human genome with fluorescent proteins. For each gene, we needed to design ...

15. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...through several different applications such as fluorescent protein fusion studies and the generation of ...

16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...

17. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    Published

    Feb. 4, 2016, 3:30 p.m.

    ...associated, such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can ...

18. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ... are enriched using either drug selection or fluorescence-based cell sorting and screened for a particular...

19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...regulation of gene expression including growth or fluorescence. There are a few examples of engineered RNA ...

20. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (...