We narrowed to 378 results for: Hal

1. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...presents itself even outside of work, consider challenging yourself, take the opportunity, and discover ...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

3. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...

4. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ... method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation’...

5. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ... to amplify away from the insertion site. Encode half the new sequence at the 5’ end of each primer, with...

6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ... include: Self-labeling tags (e.g., SNAP-tag, HaloTag, TMP-tag) – These widely used systems consist of...

7. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...functional luciferase only when fusions of the two halves of the luciferase protein interact and enable intein...

8. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   Published

   Aug. 20, 2015, 2:30 p.m.

   ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...and when they come into close proximity, the two halves of EGFP undergo folding, maturation, and fluorescence...

10. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...persisted despite our best efforts. With a little over half of our orders coming from researchers outside the...

11. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ... Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, A. R. (2024...

12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...depending on their serotype, are particularly challenging. Their efficacy in any given system can be affected...

13. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...

14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...stay tuned!  References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic...

15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...severe health conditions such as nonsyndromic holoprosencephaly where the brain fails to divide properly into...

16. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...

17. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...technology has been studied and used in assays for over half a century; the Addgene blog has an excellent primer...

18. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...

19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

20. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...was recognized by meeting organizers in their town hall.  Iterative improvement - Making Binning Singletons...