We narrowed to 716 results for: Met

...approach combines the overlapping and trans-splicing methods. The AAV encoding part A of the transgene contains...of the transgene have been seen with the hybrid method. While these fragments are likely non-functional...Frischmeyer et al., 2002). How to pick a split AAV method While the hybrid dual vector system has a slight...Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140...

...resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete...Quantitative assessment of fluorescent proteins." Nature methods 13.7 (2016): 557. PubMed PMID: 27240257. PubMed... expressed inositide binding protein domains." Methods 46.3 (2008): 167-176. PubMed PMID: 18930153. PubMed...proteins to study mRNA trafficking in living cells." Methods in cell biology 85 (2008): 273-292. PubMed PMID...

...cassette. This leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes...leave any undesired scars in the chromosome. These methods roughly fall into two categories: those that rely... a sucrase that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover...identify double crossovers. This can be an efficient method to generate precise, unmarked mutants, but it frequently...

...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy... has recently been used to study a matrix metalloproteinase: López-Pelegrín, Mar, et al. "A novel mechanism...mechanism of latency in matrix metalloproteinases." Journal of Biological Chemistry 290.8 (2015): 4728-...Christopher Marx's lab has engineered and validated a new method called FREQ-Seq. This strategy allows scientists...

...failure and problem solving. I believe that is something lacking during degree courses; they never really...the company was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on...situation, communication between departments was sometimes difficult and the solution attempted was more ...solved with good management practices, but can sometimes go unnoticed at large organizations. Another downside...

...Surprisingly, the team noticed that this editor sometimes introduced C → G edits at certain positions within...multi-step cellular repair pathway that is known for sometimes causing double-strand breaks (DSBs). The Fei-Long...common intermediate of CGBE activity, and they are sometimes processed by error-prone non-homologous end joining...context preference predictable by machine learning methods. Nature Communications, 12(1), 4902. https://doi.org...

... database. Previously developed genome editing methods using nucleases and base editors have the potential...less constrained by PAM availability than other methods. The PAM-to-edit distance can be over 30 base pairs...can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized.... Although the addition of the RNA pseudoknot sometimes altered epegRNA expression compared to pegRNAs...

...and methods section. This section will vary slightly depending on the application — flow cytometry, western...  Figure 4: A screenshot of the materials and methods section for the data report shown in Figure 3. ...

...mechanism of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’...genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903. PubMed...

...the fusions had some negative effect on these parameters. IT5 and IT6 displayed good binding efficiency...epitopes for innocuous protein tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte...protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed PMID: 23435261. Resources...

...timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech club...division of labor Serve on a committee and do something big like plan a conference Recruit many supporters...departments want to be able to tell that you know about something and are passionate about it.   Maintain a content-useful...

...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we...living animals, to explore pathways that confer metastatic potential or the ability to survive in certain...

... protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids...photostable photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids ...

...were pleasantly surprised by the efficiency of the method. One observation was an inverse relationship between...sites within a single gene using paired DSBs. This method allows for deletion screening by conventional PCR... and Bauer lab's related paper describes their methods for CRISPR design, cloning, and delivery, as well...

...by its lower resolution. The combination of this method with superresolution microscopy may improve the... a concern. Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum...organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live cell imaging of...

...residues (or more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His...His fusion proteins to be purified by single step metal affinity chromatography. Purification of His fusion...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

...Sequence tool.   DNA sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed...backbone you need for different types of cloning methods, such as restriction enzyme cloning, Gibson assembly...Ligation Calculator: This tool works for any cloning method. You can select from five different insert:backbone...

...your fluorescent protein? The type of detection method depends on what type of information you are looking...summarized some of the common fluorescent detection methods and the types of experiments they are commonly ... Biosensor Experiments Experiment  Detection Method Notes Quantification of a process Plate-based...

...about anything can disrupt your bench work and sometimes no culprit can be found…. In our lab we call this...the lab gnome. See this video where he/she/it/something is captured on camera: Unfortunately, unless...optimistically grasp at limitless potential, and who sometimes wish they could live differently than we do day...

...selecting for the loss of a gene product - usually something toxic. This gene is found on the original plasmid...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose...