...the fusions had some negative effect on these parameters. IT5 and IT6 displayed good binding efficiency...epitopes for innocuous protein tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte...protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed PMID: 23435261. Resources...

...timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech club...division of labor Serve on a committee and do something big like plan a conference Recruit many supporters...departments want to be able to tell that you know about something and are passionate about it.   Maintain a content-useful...

...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we...living animals, to explore pathways that confer metastatic potential or the ability to survive in certain...

... protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids...photostable photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids ...

...were pleasantly surprised by the efficiency of the method. One observation was an inverse relationship between...sites within a single gene using paired DSBs. This method allows for deletion screening by conventional PCR... and Bauer lab's related paper describes their methods for CRISPR design, cloning, and delivery, as well...

...by its lower resolution. The combination of this method with superresolution microscopy may improve the... a concern. Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum...organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live cell imaging of...

...residues (or more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His...His fusion proteins to be purified by single step metal affinity chromatography. Purification of His fusion...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

...Sequence tool.   DNA sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed...backbone you need for different types of cloning methods, such as restriction enzyme cloning, Gibson assembly...Ligation Calculator: This tool works for any cloning method. You can select from five different insert:backbone...

...your fluorescent protein? The type of detection method depends on what type of information you are looking...summarized some of the common fluorescent detection methods and the types of experiments they are commonly ... Biosensor Experiments Experiment  Detection Method Notes Quantification of a process Plate-based...

...about anything can disrupt your bench work and sometimes no culprit can be found…. In our lab we call this...the lab gnome. See this video where he/she/it/something is captured on camera: Unfortunately, unless...optimistically grasp at limitless potential, and who sometimes wish they could live differently than we do day...

...selecting for the loss of a gene product - usually something toxic. This gene is found on the original plasmid...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose...

...periodically beat their tails left and right in a symmetrical motion to move forward. This behavior is not ...Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow forward... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...

... hit, and especially look at the new tools and methods that have been developed. Can you think of a project...project that combines an exciting new method or approach with another in a way not done before in your... “not be shackled by your own subject - maybe something used in astrophysics can be applied to fungal ...

...The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that ...tags are frequently used for western blot, flow cytometry, and immunofluorescence . However, protein purification...tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t...

...First, it should “hook” your audience in with something that sparks their interest and makes them want...bring you and your audience - via the scientific method - to the resolution, or the ending of the story...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...

...necessary going forward. The standardization of CRISPR methods was a common thread at the meeting. The new Somatic...Fund, emphasizes that we need more standardized methods for evaluating CRISPR technologies, including the...Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently leads to high on-target editing and...

...research. Long story short, telling a good story is something that can be learned, and will serve you well in...yourself jumping backwards in time to explain something, rethink your structure. Develop your protagonist...but there are plenty of other options. Choose something with emotional significance that is likely to ...

...the pocket of a folded protein, or blocked by something bound to the protein, the antibody can't bind ...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...

...transfected with three different vectors and flow cytometry is used to sort populations by mCherry and GFP... base-editing efficiency using XMAS-TREE-based methods at various genomic loci in four different categories...Cas13Y, two compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered...

...Experimental design & methods: These questions are focused on injection and detection methods. For example, you...until performing the experiment? What detection methods did you use for expression of AAV cargo? Did you...