Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 711 results for: Met

Showing: 321 - 340 of 711 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, R...concentration changes in response to experimental parameters. This is a job for a semi-quantitative western...not especially linear. In panel C, two different methods of quantifying total protein are compared. While...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...failure and problem solving. I believe that is something lacking during degree courses; they never really...the company was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on...situation, communication between departments was sometimes difficult and the solution attempted was more ...solved with good management practices, but can sometimes go unnoticed at large organizations. Another downside...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...Surprisingly, the team noticed that this editor sometimes introduced C → G edits at certain positions within...multi-step cellular repair pathway that is known for sometimes causing double-strand breaks (DSBs). The Fei-Long...common intermediate of CGBE activity, and they are sometimes processed by error-prone non-homologous end joining...context preference predictable by machine learning methods. Nature Communications, 12(1), 4902. https://doi.org...

  4. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... database. Previously developed genome editing methods using nucleases and base editors have the potential...less constrained by PAM availability than other methods. The PAM-to-edit distance can be over 30 base pairs...can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized.... Although the addition of the RNA pseudoknot sometimes altered epegRNA expression compared to pegRNAs...

  5. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...and methods section. This section will vary slightly depending on the application — flow cytometry, western...  Figure 4: A screenshot of the materials and methods section for the data report shown in Figure 3. ...

  6. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...mechanism of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’...genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903. PubMed...

  7. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...the fusions had some negative effect on these parameters. IT5 and IT6 displayed good binding efficiency...epitopes for innocuous protein tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte...protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed PMID: 23435261. Resources...

  8. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech club...division of labor Serve on a committee and do something big like plan a conference Recruit many supporters...departments want to be able to tell that you know about something and are passionate about it.   Maintain a content-useful...

  9. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we...living animals, to explore pathways that confer metastatic potential or the ability to survive in certain...

  10. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids...photostable photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids ...

  11. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...were pleasantly surprised by the efficiency of the method. One observation was an inverse relationship between...sites within a single gene using paired DSBs. This method allows for deletion screening by conventional PCR... and Bauer lab's related paper describes their methods for CRISPR design, cloning, and delivery, as well...

  12. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...by its lower resolution. The combination of this method with superresolution microscopy may improve the... a concern. Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum...organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live cell imaging of...

  13. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...your fluorescent protein? The type of detection method depends on what type of information you are looking...summarized some of the common fluorescent detection methods and the types of experiments they are commonly ... Biosensor Experiments Experiment  Detection Method Notes Quantification of a process Plate-based...

  14. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...about anything can disrupt your bench work and sometimes no culprit can be found…. In our lab we call this...the lab gnome. See this video where he/she/it/something is captured on camera: Unfortunately, unless...optimistically grasp at limitless potential, and who sometimes wish they could live differently than we do day...

  15. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...selecting for the loss of a gene product - usually something toxic. This gene is found on the original plasmid...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose...

  16. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...periodically beat their tails left and right in a symmetrical motion to move forward. This behavior is not ...Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow forward... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...

  17. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...residues (or more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His...His fusion proteins to be purified by single step metal affinity chromatography. Purification of His fusion...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

  18. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...Sequence tool.   DNA sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed...backbone you need for different types of cloning methods, such as restriction enzyme cloning, Gibson assembly...Ligation Calculator: This tool works for any cloning method. You can select from five different insert:backbone...

  19. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ... hit, and especially look at the new tools and methods that have been developed. Can you think of a project...project that combines an exciting new method or approach with another in a way not done before in your... “not be shackled by your own subject - maybe something used in astrophysics can be applied to fungal ...

  20. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...necessary going forward. The standardization of CRISPR methods was a common thread at the meeting. The new Somatic...Fund, emphasizes that we need more standardized methods for evaluating CRISPR technologies, including the...Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently leads to high on-target editing and...
Showing: 321 - 340 of 711 results