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We narrowed to 849 results for: NTS

Showing: 321 - 340 of 849 results

  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme...and is particularly convenient for targeting experiments requiring single strand DNA generation, such ... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast... the other Cas family members make for useful implements in the scientist toolbox. If your genome engineering.... Choosing the right Cas can really put your experiments Nsync.   References and resources   References...

  2. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...ChRger. In both experiments, neurons expressing ChRgers produced stronger photocurrents at low light intensity...invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are commonly ...properties best suited for less-invasive optogenetics experiments. The sequences in this library were designed ...in vivo minimally invasive optogenetics. Both experiments delivered ChRgers by intravenous injection of...the light was turned off.  Together these two experiments determined that ChRger2’s improved light sensitivity...

  3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...guide you through the basics of CombiGEM-CRISPR experiments. CombiGEM-CRISPR: The marriage of two simple ...interrogating their functions. The continual advancements in gRNA design necessary to achieve maximal ...highly flexible and can accommodate any genetic elements of interest. It can thus be tailored to address... the combinatorial expression of other genetic elements including transcription factors (Cheng et al.,...et al., 2015). Starting your CombiGEM-CRISPR experiments The first thing you will need is to get the list...should be verified by NGS before beginning your experiments. Figure 2: Strategy for assembling barcoded...

  4. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...where to look for a coach. Many (most?) graduate students and postdocs may feel they have limited funds ...references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will have loads of success...consider. Feel free to add other suggestions in the comments or email me with suggestions.  Most coaches have...through your PhD? Some coaches focus on graduate students. For example Dora Farkas, founder of Finish Your...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...

  5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...first split into two non-functional fragments. When these two fragments are near each other, they combine...non-functional fragments: N-terminal BirA* (N-BirA*) and C-terminal BiraA* (C-BirA*). These two fragments are fused...pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting...When  proteins Y and Z interact, the two BirA* fragments complement each other to form a functional BirA...

  6. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...share, remix, store, and track these essential components of the scientific toolbox. What are Recombinant...already begun creating plasmids encoding affinity reagents. These include conventional heavy and light chain...nanobodies, ScFVs) and non-antibody based affinity reagents (e.g., monobodies, DARPins) (Helma et al., 2015...what antibody they are using and any validation experiments performed will be useful in perpetuity.  Second...CRISPR community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding... AM, Plückthun A (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038...

  7. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...questions. Story telling shares many of the same elements as a STAR answer: a setting, a conflict, and a...which the story demonstrates I possess.   Bullet points on resumes are like cliff notes, so I use these.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...professional  First impressions matter, so make sure yours counts. Dress professionally but still like yourself. ...experience less stressful. First, you can make arrangements with those you live with to have a quiet place...talk. Some video conferencing software allows participants to “raise” their hand to ask a question or type...

  8. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ... for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally from E.coli,...modified ABE7.10 accordingly which resulted in variants named “ABE8.x”: “8” stands for the eighth round...x-d.  This resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency...PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these variants, ABE8 could target many additional locations in...cells and return the corrected cells back to the patients. For example, defects in hemoglobin (𝛽-globin...continuous expression of fetal hemoglobin even in adult patients. In order to analyze the clinical use of ABE8 ...

  9. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...can be used to induce site-specific recombination events, and that the orientation of the flanking loxP ...-the FLEx switch--which utilizes recombination elements to turn off expression of one gene, while simultaneously...target sites to induce site specific recombination events: Cre recombinase binds loxP sites, while FLP binds... the DNA fragment, any additional recombination events are impossible even in the presence of Cre recombinase...wide variety of in vivo conditional gene rescue experiments, as well as for improvement of optogenetic adeno-associated...Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...prokaryotic or eukaryotic, based on the functional elements they contain. Plasmid DNA in both prokaryotic ...typically occur directly after any 3’ regulatory elements, such as the polyadenylation or poly(A) signal...Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0...terminators to reduce unwanted translation of downstream elements. A high affinity terminator may be desired for...non-poly(A) transcript-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use weaker...Gil A, Proudfoot NJ. Position-dependent sequence elements downstream of AAUAAA are required for efficient...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...was the outdoor market where one can shop for components of traditional Chinese medicine. There were hundreds...flora or fauna. I tried to find out what certain ingredients might be used for (like what would one take dried...genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when small RNA...tRNAs and tRNA-derived RNAs. Stay tuned for big developments in this field. Nobel Laureates and the ever ...furiosus, a hyperthermophile that exists in sea vents at the boiling point of water. Dr. Terns gave a ...Yat-sen University presented two new technical developments. Using the Tet-on system, his lab has created...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...can obtain known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with  Addgene. ...understand this complexity using existing C. elegans mutants. For instance, we use a mutant known as CX51 that...we have the capability to study many different mutants because the Caenorhabditis Genetics Center (CGC...model  so that we can use CRISPR/cas9 to build the mutants, analyse their motor behaviour, and ultimately ...sequencing again - it’s too inefficient and the reagents are too expensive to make it practical - but we...

  13. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...human body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak... of Queensland) to observe membrane trafficking events in vivo. Locate your protein and see who it is ...structures. In order to interpret colocalization experiments in a scientific and meaningful way it is important...are not without limitations and well-executed experiments require some consideration of FP properties. ...developed FPs optimized for diverse cellular environments. Since colocalization relies upon the detection... for fluorescently tagged viral and bacterial components. For example, the Rainbow Vectors from the Mariette...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...particular organelles and their contents, or perform in situ hybridization experiments. In many cases fluorescent...proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1: Monomeric...FP timers can be used in any situation where one wants to understand the relationship between the age of...find use in studies of animal development where events like the patterning of nascent tissues and the ... in a new and creative way? Let us know in the comments section below! References 1. Terskikh, Alexey...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...limitations to a system that relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to...with both the nuclease-positive and nuclease-null variants of Cas9 along with the necessary gRNAs, but it...gene of interest, Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the...gRNAs targeting different sites of interest thus represents a straightforward means of exerting tight control...studying biosignature preservation in Mars analogue environments.     Alejandro (Alex) Chavez is a member of ...CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    .... AAV is usually the limiting reagent for my experiments, so I always make more AAV than I think I need...than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet. My ...higher if I used PEG precipitation, but for my experiments, I get enough virus from the cell pellet alone... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...iodixanol AAV purification. If you’re new to creating gradients, practice making the iodixanol layers before doing...production tips or tricks? Please share them in the comments below! Special thanks for Dr. Dan Stone and...

  17. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...seriously damage public trust in scientists, experiments involving potential gene drives should be conducted...consequently use these precautions in their own experiments2,3. While that didn't work quite as well as anticipated4.... Ecological confinement involves performing experiments in a geographic area where escaped organisms ...be able to find mates2. For example, ongoing experiments attempting to build gene drives in tropical mosquito...flies6. It's also worth noting that gene drive experiments are less hazardous in organisms that seldom reproduce...multiplicative, and there are very few gene drive experiments that can't be performed with two or more confinement...

  18. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...number of modules. Constraints on the order of the modules, because neighboring fragments require compatible...although the specific sequences of the genetic elements may differ (e.g., a set of various mammalian promoters... to combine the functions of different genetic elements to generate non-natural tools such as novel biological...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...

  19. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...balance my daily tasks, meet cross-team project commitments, respond to any issues raised by team members...across most, if not all, industries and work environments. This is why they are called transferable skills...ask about your creativity, your potential manager wants to hear a specific time that you thought outside...boss needs someone to step up, when a new venture presents itself even outside of work, consider challenging... know you possessed. Think about your shining moments. Recall a few times you really felt great about...If you are responsible for working with lots of students, managing inventories in the lab, or organizing...

  20. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...performing these experiments. False positives can occur even in small scale experiments, although these...protein-protein interactions. In later years, improvements to yeast transformation protocols also helped... can either be generated using random genomic fragments from the organism of interest, or a defined cDNA...mating two single haploid yeast fusion plasmid transformants into a diploid strain for screening), or arrayed...both false positive and false negatives, these experiments must be set up, conducted and analysed with care...
Showing: 321 - 340 of 849 results