We narrowed to 858 results for: NTS

... to use the Gal4/UAS system, but the available reagents do not match your needs. If YFG is thrillingly...generate a transgenic fly There are a few important points to consider when choosing the vector you’d like...comparisons between lines expressing different variants of YFG because the transgenes are found in the...won’t be positioning effects). When designing experiments where you want to express multiple transgenes...chromosome your docking site is located). This amounts to a small difference of two or three weeks. 3....for. Making your transgenic fly and downstream experiments Once your gene of interest is cloned into a plasmid...

...substrates can permit measurements of luminescence from live cells. Luciferase variants with incompatible ...proteins offer different kinetics, substrate requirements, and photon emission wavelengths. In addition...analysis to investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated...binding interaction brings the two luciferase components together. A similar approach can test protein-protein... substrates can also allow multiplexing with experiments that supply both substrates and measure luciferase...

...diseases. They do that by studying patients to uncover new gene variants associated with conditions of interest...interest, then studying those variants in model systems: either human cells or mice. In a recent issue...implicated in disease or, in cases where particular variants or mutations have been reported in people, we .... You could use the kit to insert all of those variants into stem cells with the same genetic background...make induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have...

...used together in the same experiment because the reagents used to detect the presence of an antibody depends...applications for antibodies You can use antibodies for experiments in a variety of fields including neuroscience...lysates, whole cells, or tissue samples. Many experiments that use antibodies use a primary antibody, which...buffer. Then, the purified protein can be used in experiments to compare the relative amount of protein present...such as Western blots or ELISAs, or for other experiments to study protein-protein interactions. Flow cytometry...2016) Aptamers, antibody scFv, and antibody Fab’ fragments: An overview and comparison of three of the most...most versatile biosensor biorecognition elements. Biosensors and Bioelectronics 85:32–45 . https://doi.org...

...experiment work? What are the various tools and components necessary to run it? And, most importantly, what...optogenetics: 1) electrophysiology and 2) behavioral experiments. I’m not an electrophysiologist so I cannot speak...any optogenetic experiment. There are numerous variants for the different types of opsins available and...these reviews for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn...learn about the many different opsin variants and how they can be used is to consult the many primary research...in which you monitor expression at various time points (e.g. every 1-2 weeks over 8 weeks) in order to...science considerations for your optogenetics experiments. Stay tuned!  Many thanks to our guest blogger...

...ChRger. In both experiments, neurons expressing ChRgers produced stronger photocurrents at low light intensity...invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are commonly ...properties best suited for less-invasive optogenetics experiments. The sequences in this library were designed ...in vivo minimally invasive optogenetics. Both experiments delivered ChRgers by intravenous injection of...the light was turned off.  Together these two experiments determined that ChRger2’s improved light sensitivity...

...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme...and is particularly convenient for targeting experiments requiring single strand DNA generation, such ... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast... the other Cas family members make for useful implements in the scientist toolbox. If your genome engineering.... Choosing the right Cas can really put your experiments Nsync.   References and resources   References...

...ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression in a particular...genome editing, optogenetics, and chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application...available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity Empty rAAV has a cargo...vectors with multiple genes packaged in them and use elements such as IRES or 2A to co-express them from one...take the next step – selecting your AAV and AAV components (serotype and promoter) for use in your experiment...Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide ...

...guide you through the basics of CombiGEM-CRISPR experiments. CombiGEM-CRISPR: The marriage of two simple ...interrogating their functions. The continual advancements in gRNA design necessary to achieve maximal ...highly flexible and can accommodate any genetic elements of interest. It can thus be tailored to address... the combinatorial expression of other genetic elements including transcription factors (Cheng et al.,...et al., 2015). Starting your CombiGEM-CRISPR experiments The first thing you will need is to get the list...should be verified by NGS before beginning your experiments. Figure 2: Strategy for assembling barcoded...

...where to look for a coach. Many (most?) graduate students and postdocs may feel they have limited funds ...references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will have loads of success...consider. Feel free to add other suggestions in the comments or email me with suggestions.  Most coaches have...through your PhD? Some coaches focus on graduate students. For example Dora Farkas, founder of Finish Your...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...

...questions. Story telling shares many of the same elements as a STAR answer: a setting, a conflict, and a...which the story demonstrates I possess.   Bullet points on resumes are like cliff notes, so I use these.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...professional  First impressions matter, so make sure yours counts. Dress professionally but still like yourself. ...experience less stressful. First, you can make arrangements with those you live with to have a quiet place...talk. Some video conferencing software allows participants to “raise” their hand to ask a question or type...

... for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally from E.coli,...modified ABE7.10 accordingly which resulted in variants named “ABE8.x”: “8” stands for the eighth round...x-d.  This resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency...PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these variants, ABE8 could target many additional locations in...cells and return the corrected cells back to the patients. For example, defects in hemoglobin (𝛽-globin...continuous expression of fetal hemoglobin even in adult patients. In order to analyze the clinical use of ABE8 ...

...first split into two non-functional fragments. When these two fragments are near each other, they combine...non-functional fragments: N-terminal BirA* (N-BirA*) and C-terminal BiraA* (C-BirA*). These two fragments are fused...pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting...When  proteins Y and Z interact, the two BirA* fragments complement each other to form a functional BirA...

...share, remix, store, and track these essential components of the scientific toolbox. What are Recombinant...already begun creating plasmids encoding affinity reagents. These include conventional heavy and light chain...nanobodies, ScFVs) and non-antibody based affinity reagents (e.g., monobodies, DARPins) (Helma et al., 2015...what antibody they are using and any validation experiments performed will be useful in perpetuity.  Second...CRISPR community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding... AM, Plückthun A (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038...

...can be used to induce site-specific recombination events, and that the orientation of the flanking loxP ...-the FLEx switch--which utilizes recombination elements to turn off expression of one gene, while simultaneously...target sites to induce site specific recombination events: Cre recombinase binds loxP sites, while FLP binds... the DNA fragment, any additional recombination events are impossible even in the presence of Cre recombinase...wide variety of in vivo conditional gene rescue experiments, as well as for improvement of optogenetic adeno-associated...Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

...prokaryotic or eukaryotic, based on the functional elements they contain. Plasmid DNA in both prokaryotic ...typically occur directly after any 3’ regulatory elements, such as the polyadenylation or poly(A) signal...Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0...terminators to reduce unwanted translation of downstream elements. A high affinity terminator may be desired for...non-poly(A) transcript-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use weaker...Gil A, Proudfoot NJ. Position-dependent sequence elements downstream of AAUAAA are required for efficient...

...was the outdoor market where one can shop for components of traditional Chinese medicine. There were hundreds...flora or fauna. I tried to find out what certain ingredients might be used for (like what would one take dried...genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when small RNA...tRNAs and tRNA-derived RNAs. Stay tuned for big developments in this field. Nobel Laureates and the ever ...furiosus, a hyperthermophile that exists in sea vents at the boiling point of water. Dr. Terns gave a ...Yat-sen University presented two new technical developments. Using the Tet-on system, his lab has created...

...can obtain known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with  Addgene. ...understand this complexity using existing C. elegans mutants. For instance, we use a mutant known as CX51 that...we have the capability to study many different mutants because the Caenorhabditis Genetics Center (CGC...model  so that we can use CRISPR/cas9 to build the mutants, analyse their motor behaviour, and ultimately ...sequencing again - it’s too inefficient and the reagents are too expensive to make it practical - but we...

...human body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak... of Queensland) to observe membrane trafficking events in vivo. Locate your protein and see who it is ...structures. In order to interpret colocalization experiments in a scientific and meaningful way it is important...are not without limitations and well-executed experiments require some consideration of FP properties. ...developed FPs optimized for diverse cellular environments. Since colocalization relies upon the detection... for fluorescently tagged viral and bacterial components. For example, the Rainbow Vectors from the Mariette...

...particular organelles and their contents, or perform in situ hybridization experiments. In many cases fluorescent...proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1: Monomeric...FP timers can be used in any situation where one wants to understand the relationship between the age of...find use in studies of animal development where events like the patterning of nascent tissues and the ... in a new and creative way? Let us know in the comments section below! References 1. Terskikh, Alexey...