We narrowed to 479 results for: RET
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TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....“These novel methods will be instrumental in interpreting human variation at large scales and making personalized...
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Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m.... mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying a reagent... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....time to network,” Zhang says. “The schedule was pretty busy, but after the meeting, I received a lot of... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m.... nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate untranslated mRNAs... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....highly optimized bioinformatics pipeline that will return high-quality assembled and circularized plasmids... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....consider hfYFP and LSSmGFP. Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....recommendation for the correct statistical test can save a retraction. Today, collaborations are limited by the difficulty... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....fluorescent signals upon sensing glutamate in mouse and ferret models. Furthermore, they created three affinity... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer lentivirus... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....transgene is flanked by two ITRs, and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....force-generating protein machines that can extend and retract over many microns in response to pH changes without... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....repeated elements “direct repeats” and the unique stretches of DNA between them “spacers” (see figure below... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....proteins, imaging four colors simultaneously should be pretty straightforward. Thank You to our Guest Blogger... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....dye! Always run a ladder. Ladders allow you to interpret the bands that you get in your sample lanes. Choose... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m....CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m.... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ...