We narrowed to 459 results for: SHI
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TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....as secondary antibodies. sdAbs, however, really shine in vivo, like “chromobodies,” which are single-domain...
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Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....proposes promising avenues for future engineering to shift the spectra and improve the performance of HcKCRs... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma from the... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog PostPublishedNov. 17, 2015, 3:30 p.m....also organises PhD information events and summer internship information sessions to help undergraduate students... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....regaled us with tales of back country skiing, fly fishing, and local grizzly bear attacks. He described one... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....After joining a lab, the focus shifts to choosing an unexplored and impactful research topic that aligns... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....CRISPR/Cas technology? Let us know. References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1 inducible CMV promoter and targeted to a sequence... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....immobilization on a solid support. This allows washing away of unbound material to calculate the amount... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....what the user wants to do and make the path to accomplishing their goals as intuitive and painless as possible... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....Pennsylvania University of Utah University of Washington Vanderbilt University and Medical Center Weizmann... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....reprogramming outcomes. CellTag from the Morris Lab at Washington University in St. Louis provides a combinatorial... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....and passion on display in our building, each day brushing past and chatting with individuals whose research... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, you can then apply your... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 ... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....logical choice for efficiency. Where viruses really shine is when a specific cell type within a live organism... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m.... we’ve experienced incredible growth with our readership expanding from ~10,000 users per month in November... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....heterologous genes, but the trend of research has shifted in recent years toward tinkering with regulatory... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m.... in addition to preparing DNA and generating/replenishing kits, is to find new ways to make lab processes...