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We narrowed to 581 results for: SIM

Showing: 321 - 340 of 581 results

  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... in different locations all over the world. But simply attending the conference is usually not enough....young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face...

  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...healthcare consulting firms can be involved in many similar activities depending on the expertise of the senior... to a community of colleagues who are pursuing similar jobs and non-academic careers. Now, more than ever...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...work in cell culture. The problems with cell lines Simply put, the majority of cell line problems come from...monitor any changes. Your tracking system could be as simple as a piece of paper on the front of the incubator...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...approach the situation differently, some adopting simple note-taking tools, others using excel sheets or...

  7. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...

  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...cell spent passing through the laser beam. A is simply the area under the resulting curve. By default,...for both markers. When comparing two markers simultaneously, we commonly describe the resulting populations...

  9. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...

  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... by a ribonuclease called Dicer. In a great simplification of the process, Dicer chops off the loop of...t always cleave the mRNA — the bound miRISC can simply block translation machinery. The composition of...

  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...need to know the exact quantity of your POI, but simply whether its concentration changes in response to...controls are equally strong, so the normalized value simply reports which POI band is stronger. The right gel...

  12. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...

  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

  14. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...

  16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

  17. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need.  Prioritizing resources in your budget The simple practice of putting together a resource budget ...

  18. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...advantages and disadvantages. Sanger sequencing is simple and the most accurate method as it directly reads...predominant state of registers in a sample based on a similarity metric that we defined in our paper. In our opinion...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...
Showing: 321 - 340 of 581 results