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Showing: 321 - 340 of 571 results

  2. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

  3. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...approach the situation differently, some adopting simple note-taking tools, others using excel sheets or...

  5. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need.  Prioritizing resources in your budget The simple practice of putting together a resource budget ...

  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

  9. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...advantages and disadvantages. Sanger sequencing is simple and the most accurate method as it directly reads...predominant state of registers in a sample based on a similarity metric that we defined in our paper. In our opinion...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... and anterograde transport, but they also have similar transport characteristics (velocities of movement.... 3. Aschauer, Dominik F., Sebastian Kreuz, and Simon Rumpel. "Analysis of transduction efficiency, tropism...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar...because they had close relatives suffering from similar diseases. Others were inspired by the imminent ...

  14. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...Mismatch Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...CD45 expression turns out to be weak. Table 2: A simple flow panel to detect live, transduced, and CD45...reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here...

  17. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...://doi.org/10.1371/journal.pone.0016765. Moore, Simon J., et al. “EcoFlex: A Multifunctional MoClo Kit...

  18. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...discussed below. The software could also be used as a simple tool to rapidly find one or a few gRNAs suggested...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...

  19. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

  20. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...
Showing: 321 - 340 of 571 results