We narrowed to 354 results for: Sele

1. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...general approach for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus...

2. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   Published

   Aug. 5, 2014, 6:51 p.m.

   ...someone other than the actual author. Instead, selected images are said to be representative or the statistical...

3. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...carefully review your options. If possible, try to select a couple of antibodies that seem promising to compare...

4. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ...directed-evolution methods, the activity-based selection in mammalian cells used here could be an attractive...

5. March for Science

   Type

   Blog Post

   Published

   April 21, 2017, 2:30 p.m.

   ...information for your senators all you need to do is select your state. State Legislatures: To find the website...

6. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...insertions/deletions, therefore making those alleles useless for our studies. If one now also takes into account...

7. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...performing probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...requires less biotin, and allows scientists to more selectively target fusion proteins to specific locations ...

9. Site Directed Mutagenesis by PCR

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   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...from the parental plasmid provides a mean for selecting for transformants which have taken up the mutagenized...

10. Evolution of Lab Techniques

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    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...still be used to screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels ...

11. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    .... Cre production drives recombination and XFP selection, followed by Cre self-excision. These constructs...

12. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...worked hard designing your plasmid – you carefully selected the optimal promoter for your gene of interest...

13. A Control for All Seasons

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    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ...101: Choosing the Right Isotype Antibodies 101: Selecting the Right Antibody Resources on Addgene.org Addgene's...

14. Plasmids 101: Dimers and Multimers

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    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ...although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid Multimers...

15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen’s lab also ...

16. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ...regulate gene expression) that uniquely express selectable and/or sortable reporters in certain states of...

17. Plasmids 101: Restriction Cloning

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    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ... other materials pass through; and, finally, selectively elute the plasmid DNA from the column using a...

18. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...suggested important things to emphasize in order to be selected in foreign labs.” Elena Grosso (Mentee IMP 2014...

19. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...Still trying to choose an antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies...

20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful...