We narrowed to 816 results for: ache
-
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....Typically, there is a very small number of ASCs in each tissue and once removed from the body; their ability...generating iPSCs iPSCs and ESCs closely resemble each other in many aspects. Like ESCs, iPSCs have a high...this series, we’ll discuss the two widely used approaches of somatic cell conversion: conversion via iPSC...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....
-
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....this later). Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well as...sensing to signal output slightly slower than other approaches. Functionally, the voltage sensitivity of the...level in vitro. In vivo monitoring has also been achieved with these sensors, but further optimization is...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m.... to linking pieces of DNA that are far away from each other in terms of base pairs, but close together...method is that a new cell line has to be created for each genomic loci of interest. This is cumbersome and...analyzed with CAPTURE 2.0, with two gRNAs targeting each site. They found that DNA sequences targeted by ...to interact with, and how many promoters or genes each enhancer is interacting with. Super enhancers are... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m.... when I first became a manager. Know your people Each person is unique and should be managed accordingly...writing a grant, thinking about the next project, reaching out to other teams, expanding your network…. I...problem, I was able to resolve this issue - with coaching - before it became a major problem. But I also...always the case or you may not feel comfortable reaching out to your manager. This is when having a mentor... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m.... instances of focused attention become magical teaching moments. The models function as thinking tools...instructional materials that resulted from our work with teachers, and to create custom models of proteins for researchers...that the resulting physical model is an effective teaching tool. You can read about Jacob’s initial reaction...BioMolecular Modeling web site to check out our hands-on teaching tools that are designed to make the molecular ... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....described below (See Figure 1). Figure 1: Each problem with dimerizing fluorescent proteins is ...increasing the probability that two FPs will collide with each other. The consequences can be dramatic. For a membrane...the Endoplasmic Reticulum and live cell imaging approaches. Erik is also an avid long distance runner, gardner...14581454. PubMed Central PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m.... screening), or arrayed on a 96 well plate where each well contains a defined prey fusion. The former...possible to screen libraries of bait and prey against each other at the same time in order to try and access...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, ... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...noting that it is possible to design a vector that achieves persistent transgene expression in certain cell...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl, H. C. J. (2007). Adenoviral... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....neurons and GFP (green) to label neurons transduced by each serotype. Working at Addgene has shown me this... AAV vectors from Addgene and the CLOVER Center, each one submitted by researchers like you. Reports are...only be effective if many scientists choose to help each other by sharing their data. So take advantage of...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m.... primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which are summarized... your plasmid Have to make a new primer pair for each insert Backbone-Specific Provides information...cloning You have to make a new primer pair for each insert since primers are insert dependent ... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....salaries). Postdocs (who are not in a primarily teaching role) come under this ruling, regardless of visa...on higher education, you can see that those in teaching roles (such as adjuncts) and graduate and undergraduate...policies. It is unclear how many postdocs are at each of these institutions (populations potentially from...trainee workforce has clearly not been a sustainable approach to biomedical research in the U.S. and postdoctoral... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein degraders. But CRBN...images of single focal planes through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our... -
Supporting Reproducibility with a Connected ELN
TypeBlog PostPublishedJan. 17, 2019, 1:38 p.m....are many types of electronic lab notebooks (ELNs), each with its own pros and cons. All ELNs have the virtue... take a look at the RSpace ELN. The connected approach in action: how the RSpace ELN supports reproducibility...research, and data repositories. Below I describe how each of these kinds of connectivity facilitates reproducibility...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....required to develop new projects and test novel approaches, a necessary skill set for any scientist. In ...lab Thinking Outside the Box: Individual Approaches Whether it’s finding the source of contamination...your situation. Thinking Outside the Box: Team Approaches At some point or another, you’ll find yourself...benefits from their policy! Perfectionism When we reach for perfection, our work stalls. We become so fixated... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....using standard methods that have been optimized for each specific virus in order to generate high quality... generally two to three fold higher than those achieved using the standard qPCR method. Titering by the...The specific QC experiments performed varies for each viral lot. To learn which specific QC experiments... throughout production, ensuring the identity of each sample from the DNA tube all the way to the viral... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....are not general amplification protocols. Rather, each protocol is optimized for a single, specific library...step also depends on the individual library. So, on each library’s page, you can find protocols for sequencing...protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T cells...virus–the right amount of virus, that is. Ideally, each cell will be transduced by no more than one viral... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m.... work my lab wanted to do already seemed to know each other and what to do. Saved by the Tweet - The...people attending large meetings. A key was to provide each bin with a mentor who had attended the meeting multiple...networking and mentoring circles We looked at a lot of approaches scientific meetings have taken to provide mentoring...Joe taught high school chemistry for a year. The teaching/mentoring bug never went away. While he has frequent... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....primers for each individual sample, this will reduce convenience and comparability because each sample with...other DNA quantification methods - see below), since each viral particle typically contains one viral genome...indication of infectious titer, consider titrating each lot of AAV in vivo to determine the optimal dosage... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....rewarding parts of the job. Solutions may also be achieved by simply replacing the product in question. This...different backgrounds and realizing that the same approach does not work for everyone. The benefit is that... in project management, consulting, scientific outreach, and more. Technical support roles also involve... images used in this post. Klaus Wanisch is an Outreach Scientist at Addgene. These days he's doing a ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....recombinase have been developed. A common means to achieve ligand control is to fuse a mutant estrogen receptor...be transfected by in utero electroporation. To achieve sparse labeling of individual neurons one could...the same time from two different plasmids this approach becomes problematic as it reduces the probability... the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently to DNA via a Phosphotyrosyl Linkage...