We narrowed to 830 results for: ache

...the genome. Native Cas9 has two catalytic domains, each of which cleaves one strand of DNA upon binding ...proteins derived from different bacterial species, each of which interacts with a distinct gRNA that allows...expression with truncated gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying...engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826. PubMed ...

... to linking pieces of DNA that are far away from each other in terms of base pairs, but close together...method is that a new cell line has to be created for each genomic loci of interest. This is cumbersome and...analyzed with CAPTURE 2.0, with two gRNAs targeting each site. They found that DNA sequences targeted by ...to interact with, and how many promoters or genes each enhancer is interacting with. Super enhancers are...

... of the post! Speed: Your grant deadline is approaching or your advisor is demanding results. If you’...Cost: Funding is usually limited - what cloning approach should you use if the budget is particularly tight...however, or simply looking for a more streamlined approach, you might want to give Gateway a try. This method...answer, so don’t be afraid to explore multiple approaches to find out which one works best for you. Happy...

... when I first became a manager. Know your people Each person is unique and should be managed accordingly...writing a grant, thinking about the next project, reaching out to other teams, expanding your network…. I...problem, I was able to resolve this issue - with coaching - before it became a major problem. But I also...always the case or you may not feel comfortable reaching out to your manager. This is when having a mentor...

...to, I made it a personal goal to ask one question each day (this may be more or less difficult depending...identifies a potential collaborator from the meeting, reach out to them, pointing out that you heard their talk...handles to help you find other scientists attending. Reach out if you’d like to meet someone and set aside ...to set goals for myself like meet ten new people each day. Exchange business cards. When you’re done networking...

... the human genome with fluorescent proteins. For each gene, we needed to design guide RNAs, donor templates...gaussian normalized cut-to-insert distance) This gives each guide a score ranging between 0 and 1. The cutting...target. The CFD score ranges between 0 and 100 for each guide, with 100 being the strongest interaction ...but the protein sequence they encode is not. After each mutation, it will check the CFD score. If it is ...

...Genentech, will not. The point is that each company has a unique approach, and some might suit your needs better...setting—especially if job applications require teaching experience or high numbers of publications, which...into the general careers section of their web site. Each position is for a specific, pre-approved project...

...Typically, there is a very small number of ASCs in each tissue and once removed from the body; their ability...generating iPSCs iPSCs and ESCs closely resemble each other in many aspects. Like ESCs, iPSCs have a high...this series, we’ll discuss the two widely used approaches of somatic cell conversion: conversion via iPSC...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

... instances of focused attention become magical teaching moments. The models function as thinking tools...instructional materials that resulted from our work with teachers, and to create custom models of proteins for researchers...that the resulting physical model is an effective teaching tool. You can read about Jacob’s initial reaction...BioMolecular Modeling web site to check out our hands-on teaching tools that are designed to make the molecular ...

...described below (See Figure 1).   Figure 1: Each problem with dimerizing fluorescent proteins is ...increasing the probability that two FPs will collide with each other. The consequences can be dramatic. For a membrane...the Endoplasmic Reticulum and live cell imaging approaches. Erik is also an avid long distance runner, gardner...14581454. PubMed Central PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified ...

... is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques ...promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic vectors simultaneously... that only a single translation event occurs for each mRNA. However, some bicistronic vectors take advantage... some of these peptides, no consensus has been reached on which peptide works best. Likely the choice ...

...B-D represent the pure DNA spectra for comparison. Each of these contaminants will have a slightly different...that you use the same solution for blanking your machine!  Finally, many NanoDrop systems now provide full-spectrum...are no substitute for sound techniques, routine machine maintenance, and critical interpretation of the...are, of course, more considerations and other approaches for measuring DNA quantity and quality than we...

...transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...noting that it is possible to design a vector that achieves persistent transgene expression in certain cell...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl, H. C. J. (2007). Adenoviral...

...neurons and GFP (green) to label neurons transduced by each serotype. Working at Addgene has shown me this... AAV vectors from Addgene and the CLOVER Center, each one submitted by researchers like you. Reports are...only be effective if many scientists choose to help each other by sharing their data. So take advantage of...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,...

... primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which are summarized... your plasmid Have to make a new primer pair for each insert Backbone-Specific Provides information...cloning You have to make a new primer pair for each insert since primers are insert dependent   ...

... screening), or arrayed on a 96 well plate where each well contains a defined prey fusion.  The former...possible to screen libraries of bait and prey against each other at the same time in order to try and access...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, ...

...and organizations, like the Allen Institute, to achieve greater outcomes together. The Allen Institute... well as the strength and density of labeling in each. This allows users to quickly find the tools that...Daigle, T. L., Sedeño-Cortés, A. E., Walker, M., Kalmbach, B., Lenz, G. H., Morin, E., Nguyen, T. N., Garren...Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead...

...process relies on “sequencing-by-synthesis,” where each base is recognized and recorded as a DNA strand ...3). It uses (aptly named) “nanopores” that are attached to the flow cell membrane and have an electrical...pulled through the pore via motor proteins. When each nucleotide passes through the nanopore, the electrical...bioinformatic analysis. At Addgene, we use a mix-and-match approach for sequencing methods and choose the best method...

...ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein degraders. But CRBN...images of single focal planes through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our...

...using standard methods that have been optimized for each specific virus in order to generate high quality... generally two to three fold higher than those achieved using the standard qPCR method.  Titering by the...The specific QC experiments performed varies for each viral lot. To learn which specific QC experiments... throughout production, ensuring the identity of each sample from the DNA tube all the way to the viral...