...things. Now, work from Connie Cepko’s lab allow researchers to activate transcription or Cre recombinase ...activation The project began with a frustration many researchers have experienced: wanting to ask and answer experimental...not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to express a variety of ...and we’re eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...secondary antibodies would save a good deal of it. Researchers are continuously looking for ways to improve ...providing needed flexibility in assay development. Researchers who understand how and why to use secondary antibodies...

...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...commonly, antibodies of the IgG isotype are used for research purposes. Isolating polyclonal antibodies from...antibodies are particularly useful for certain research applications. Many secondary antibodies are polyclonal...Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...

...responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences, I ... is a style match for your needs can take some research.  It helps to spend some time thinking about what... Academic Labor Environment from the Future of Research Symposium Resources on the Addgene Website Check...Recent Job Openings Find Plasmids to Expedite Your Research   ...

...circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...which is why MoClo is so attractive! It allows researchers to assemble and test many variables all at once...is postdoc in Peter Schultz’s group at Scripps Research, where he works on genetic code expansion.  References...

...(Bradbury & Plückthun, 2015a, b). Therefore, researchers from over 100 scientific institutions have proposed...scientists can now improve genetically-encoded research tools, it is shocking that the plasmids and sequences... (2012) Raise standards for preclinical cancer research. Nature 483:531–533 . https://doi.org/10.1038/...Reproducibility: Standardize antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a...

...removing the wild-type TadA from the complex the researchers developed a second set: The ABE8.x-d version...3.2x higher for positions A3-A4 and A8-A10. The researchers selected 8 of the tested ABE8 constructs (ABE8.8... base editing.  Try ABE8 base editors for your research! Base editing in primary human cells  Cell therapy... of ABE8 for primary human cell editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells...

...required a one-hour presentation about a prior research project. In total, the interviews lasted from ...prepared with questions to ask. With a quick Google search for “questions to ask an interviewer,” you can ... they will ask you to present about your prior research. This is a chance to highlight your prior accomplishments...deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science...

...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical... AAV vectors evade host immunity. While basic research and other ongoing clinical trials support the ...Silva Feelixge. Harshana S De Silva Feelixge is a researcher whose work has focused on gene therapy for viral... She is particularly passionate about HIV cure research and science communication.     References 1. Gaspar...

...Laboratory. Are you a science student or early career researcher looking to break into science communication? ...non-scientists in an unusual setting: Discuss your latest research in a local pub at a Pint of Science event. Or ...gives training in media skills, writing about your research and residential communication. If science journalism...'d like to directly involve the public in your research projects, you can set up a citizen science project...

...fluorescent tool with magnets would enable new research and applications that don’t yet exist, but it ... this was even possible. There is a variety of research on whether and how organisms respond to magnetic... non-biological conditions. So, they decided to search for a magnetic response among other proteins that...some hypotheses. They are excited to see other researchers from a range of fields, like quantum chemistry...

...Culture Re-Amplification Protocol (PDF, 71 KB) Barcode Watermelon Library 5,000,000 30 bp At least... Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR Pooled Libraries 200,000–1,200,000 ...Library 3,548 250 - 10,000 bp, plus a 24 bp barcode 250–1,000 ng Solid Joung et al. Nat Protoc...Pooled Library 80,962 1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification...

...to monitoring localization; using FP timers, researchers can look at a single image of a cell and understand... production of the initial FP timer in 2000, researchers have realized how problematic aggregation-prone...expression. In a developing embryo, for example, a researcher could determine whether or not newly synthesized...synthetic biology, FP timers might be able to tell researchers whether old or new cells are better at producing...

...luminescence have been widely used in biological research for a long time, chromoproteins are just now being...history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history. In 1987... can be used, why not add some color into your research? If you have a favorite chromoprotein, either ...Addgene.org Find fluorescent proteins for your research Read our Molecular Biology Reference Find plasmids...

...experiments that work anywhere in the genome. Aneesh's research interests include proteomics, machine learning...Taking a nerdy perspective, you might denote your search as follows: (my_house + coffee) - starbucks See...Data. Quilt is a collaborative database. Aneesh's research interests span machine learning, proteomics, and... on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find Validated gRNAs...

... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...

...with any scientific research, choice of a technique often comes down to the researcher’s familiarity with...the nature of the experiment, it is up to the researcher to determine which method is most appropriate...stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...

...data of their users readily accessible via web searches; likewise, most in-person events and venues will...understanding of their audience demographics. Do your research so you can go to where your audience already is...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com...since we live in the digital age, make sure to research any software you may need: cost or freeware alternatives...

... Resources on Addgene.org Search for AAV Vectors to use with your research. Visit Our AAV Guide Page ...a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating... new AAVs can serve as tools for basic science research (neuronal tracing and morphologic studies), as...

...proteins included in different CGBE tools allow the researcher to direct the repair of the abasic site. But ... originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...transversion editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing...existing CGBEs. This distinct approach means researchers can now select the best tool for their application...