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Showing: 321 - 340 of 678 results

  2. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...

  3. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...quickly. There are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been...plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that have helped scientists... of the Addgene community. Perhaps you’ll find plasmids and new tools that will speed your science along...lab closures, we’re expecting to reach ~14,000 plasmids deposited this year once they all get processed...

  4. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...Christopher E. French. Dr. Duedu was recently awarded plasmids from Addgene as a successful applicant to the ... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian...out our Microbiology Pages Find Synthetc Biology Plasmids Find Molecular Biology Resources ...

  5. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...phage pL promoter.  It is a good idea to choose plasmids that also express the repressors associated with...needed. This approach is best suited for modifying plasmids or BACs because it results in stable integration...restriction enzyme digest can be used to screen plasmids for the appropriate mutations.  Point mutations... Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods...Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains Check Out Other Molecular Biology ...

  6. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment...toward giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing...sequences and characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists...

  7. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...collection of popular plasmid sequences. These plasmids contain features such as antibiotic resistance... boundaries for control elements in commercial plasmids were inconsistent and sometimes clearly wrong....work to fill any common annotation gaps in the plasmids available through the Addgene website. In addition...plasmid analysis powered by SnapGene Browse All Plasmids ...

  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...

  9. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found...

  10. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...ll work with you to get the word out about your plasmids and their potential educational uses. We believe...30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional ...developmental biology Use the pathways over time plasmids to engage students in functional genomics research...out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here ...

  11. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...19213877 PMCID: PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation...1367-71. PMID: 25792328 PMCID: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, ...976-89. PMID: 27153498 PMCID: PMC4889334 Find plasmids from this paper at Addgene. Additional Resources...Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out Other Hot Plasmid Technologies ...

  12. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...produced R-bodies that could extend at different pHs. Plasmids expressing expressing both wild type and mutant...acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, ...

  13. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...their required components are very similar. While plasmids containing virus-specific elements such as the...serotypes (12 naturally occurring and more synthetic capsids are routinely being developed) to choose from, ...Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S., et al. Adenovirus-Mediated Gene...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

  14. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent...embryonic stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter...Zhao T-T, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB (2017) A stable but reversible ...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find resources and software ...

  15. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...that Addgene provides is an open repository of plasmids and other necessary information that’s required...the collection, storage, and distribution of the plasmids and their ability to identify and fulfill a need... helping the scientific community manage those plasmids so scientists aren’t bogged down with distributing...continuing to look at providing other things besides plasmids to the scientific community through Addgene’s ...

  16. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...different plasmids. Harvard’s DRSC/TRiP Functional Genomics Resource Center has various plasmids for overexpression...filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic...Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

  17. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited...

  18. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...

  19. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...reporter plasmids Find photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection...several advantages over existing methods. First, it avoids activating other signaling pathways, which frequently...photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here. In addition, it could be...

  20. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ..., 6175 (2023). DOI:10.1038/s41467-023-41829-y (Plasmids available here) Walton, R. T., et al., Unconstrained...368,290-296(2020). DOI:10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered...and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep 7;49(15):8785-8795. doi: 10.1093/nar... on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...
Showing: 321 - 340 of 678 results