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Showing: 321 - 340 of 650 results

  1. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments... reaction to understand if any of your buffer components could affect the reaction. For instance, sodium...

  2. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...antibody isotypes important? To your immune system, an antibody’s isotype is important because it determines...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... terms.  Weighting We determined the relative importance of different bits of information for a given ...that make our new search engine unique. Most importantly, with this new implementation, we laid the foundations...

  5. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...function in terms of its regions. But equally important is the way the brain connects across these regions...is connected to itself across these regions is important for understanding how the brain functions. The...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...they should be. Don't rush the process: It is important to give your plates enough time for any intact... susceptible to or dependent on certain media components, and are rescued by genes supplied on the transformed...

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways...chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous when ...

  9. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...requires several considerations. First, it is important to establish the type of audience you want to ... your paper to your preferred  journal, it is important to check the journal’s guidelines to authors and...

  10. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...considerations of the laboratory make object composition a smarter choice than inheritance. Some containers...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

  12. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...control of kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic ...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...

  13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...Postdoc Wages Read about the Advocating for Science Symposium and Workshop Learn about a Variety of Science ...

  14. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity Empty rAAV has a cargo...take the next step – selecting your AAV and AAV components (serotype and promoter) for use in your experiment...

  16. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ... to the microenvironment itself. They deliver compounds like proteins and nucleic acids from cancer cells..., the microenvironment backstage play just as important of a role if not more.  Many thanks to our guest...

  17. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...inverse orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

  18. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...As a scientist, perhaps the most important part of your research is communicating the results of the ...the work. Yes, those control experiments are important - but without letting other scientists know the results...

  19. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...interest. See figure 1 below for details on the key components of RaPID. Ramanathan et al. also tweaked BirA...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...fragment, any additional recombination events are impossible even in the presence of Cre recombinase. This...in the absence of the endogenous gene. Spatio-temporal expression of optogenetic reporters: Since most...
Showing: 321 - 340 of 650 results