Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 745 results for: nes

Showing: 321 - 340 of 745 results

  1. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...and prepare business cards. You might not consider being a student or postdoc a “business,” but having...having business cards is a good way to share your information quickly. During the conference Arriving at the...myself like meet ten new people each day. Exchange business cards. When you’re done networking for the day...

  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...H840. To ensure that the clones were capable of cleavage, they tested the clones against an NNN PAM library...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...variants recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition...

  3. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... do everything as I planned and I was missing deadlines - which puzzled me because I could do all these...basis! The silence and maybe even the slight awkwardness can spur people to speak up. Once aware of the...to be!  References Becoming the boss. Harvard Business Review, January 2007. Three Rules for Managing...  Are Good Managers Born or Made? UPenn Wharton Business School Blog Additional Resources on the Addgene...

  4. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...wearing a lab coat, but instead by people wearing business suits discussing multimillion dollar contracts... or coursework. First, you have to develop an awareness of when you are negotiating. Then take a moment...situation instead of just extra work for them? As a business development analyst, I negotiate with global organizations...professor or signing contracts when working for a business development team, negotiation is a key transferable...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat severe...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue their work by...CRISPR-based lineage tracing tool captures rare clones that represent 0.001% of the population.  Because...CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression ...

  6. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...cells by the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...C.A., et al., Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med, 2004. 350(...Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.... Yu, J., et al., Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007....

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown... al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue),...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...

  8. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully ...used over and over again in combination with novel genes to get very precise expression levels. The BIOFAB... for someone to forward engineer a system of 10 genes. You can now forward engineer a 10-gene system to...our testing of these parts that the chance your genes will be expressed at the level you want is 93 percent...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary... depending on whether the heavy and light chain genes are contained to one plasmid or if they’ve been ...have multiple contaminating bands or a few strong ones, consider increasing the number of washes performed...

  10. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells 23.4 ...technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed PMID: 26848818...interactions between genomic regions by enCh IP‐Seq." Genes to Cells22.6 (2017): 506-520. PubMed PMID: 28474362...

  11. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...Easy to host guests remotely. Easy access from smartphones with low data requirements.  No visuals. Variety... audience interpretation. Infographics Low Combines both words and images; especially useful for conveying... to be staffed. Mixed Media Medium to high Combines pros of multiple channels/balance out cons. Flexible...in multiple ways from the same experience.  Can combines cons of multiple medias. Switching from one media...

  12. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...of the discussed topic, and level of general busy-ness will most likely the primary drivers of frequency...attendance slumps below a certain level or interest wanes off such that attendees are not actively participating... with any recurring meeting, a journal club’s usefulness should be continually evaluated and assessed ...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Thus, contour lines that are close together show a high density of cells, while contour lines that are spread...can also be presented as contour plots. Contour lines, which you may have seen on elevation maps, are ... of cells (Figure 7).  Figure 7: Contour lines in flow data from Jhunjhunwala et al., 2015. Used...

  14. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...with the development of methodologies to deliver genes to the CNS have recently made it much easier to ..., and the long-term expression of AAV delivered genes. AAV serotypes have been extensively studied in ...experiments with tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis...been used to visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons which ...

  15. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...ice.synthego.com. We rigorously evaluated the effectiveness of the ICE tool by analyzing thousands of edits...performed over multiple experiments and comparing the robustness, accuracy, and speed with existing tools. For..., ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency (...green (edited sample) and orange (control sample) lines should be close together before the cut site. A ...

  16. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...become popular. There are only so many methods and machines one team can learn so linking together expert ...external connection is to make sure you keep open lines of communication with colleagues who move on to ...conference attendance, and I scout for potential business partners. I am glad that I was able to build experience...hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual Property...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... and Cas13 systems in a range of mammalian cell lines. However, in those same comparisons, the AbuGoot...using Cas7-11. This included tests done in cell lines known to be more sensitive to toxicity from RNA ...activity. They now had a system that could integrate genes up to 36 kb in length, with an integration rate ...primary cells, and can be easily programmed for new genes. In comparison with other large insert techniques...

  19. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...inspiration  I look back at the meeting with such fondness, knowing that, as a 38 year-old with many years... that only appear once) in our analysis. Many pipelines toss these singletons out, and there is healthy...Finding mentors for the meeting Just like analysis pipelines for DNA that group similar sequences together ...bacteria of different evolutionary lineages share genes that can improve chances of survival for an individual...

  20. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... you have these rules (word limit, formatting guidelines, etc.) you have some criteria to shape the document...To find reviews, I just searched online and found ones that I thought “looked good” by no definitive criteria...This could be in the form of lists, streams of consciousness, or anything else. Like I mentioned, I also ...T-cell development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID: 26124919...
Showing: 321 - 340 of 745 results