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Showing: 321 - 340 of 617 results

  1. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ... or have a simple pathology. In these cases, preliminary identification of potential targets by CRISPR...researchers to select and generate physiologically relevant models. In complex diseases, it can aid in generation...

  2. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...traps? Traps – gene, enhancer, and promoter – all rely on the random integration of a reporter construct... effective tools but neglect some biologically relevant situations. Tissue-specific genes – the genes...

  3. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...LitSuggest Tool Following the “rabbit hole” of related cell lines when annotating a cell line Community... helpful information on the known features and relevant literature, or to decide which line is best for...

  5. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that..., Susanna, Tenisha, Thomas, Tony, Tracy, Tyler, Tyrel, Wogenie, and Xuan Additional Resources on the Addgene...

  6. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...'s more than 50 antibodies target neuroscience-related proteins, but we recently added several broadly...into your protein of interest and recognized by reliable antibodies. It is derived from the C-terminal ...

  7. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...there are so many bad managers? New managers are rarely chosen because they have demonstrated skill at ...better with some variety in their job. Good relationships with clients and colleagues Sometimes this means...

  8. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...chimera may have different binding properties (Morelock 1994). While in the majority of cases the differences...doi.org/10.1016/b978-0-08-097037-0.00017-8     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson,...

  9. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...scheduling Diverse opportunities to do science related jobs that require no bench work Better resources... important first step. Developing mentoring relationships, especially with peer mentors, to discuss your...

  10. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

  11. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...   Biological Processes  If you’re feeling relieved that you’re working with native proteins instead...instead of denatured ones - well, don’t relax just yet. Proteins often change shapes and epitope accessibility...

  14. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...determine if our tubes were permeable to the CO2 released from dry ice, and whether this affected the pH...tested whether venting the tubes prior to thaw released the CO2 from the headspace, thus mitigating the...

  15. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...coming soon! These vectors listed below should be released at Addgene in the next 6 months, pending quality...

  16. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...give researchers the ability to monitor glutamate release from neurons and other brain cells in vivo. Glutamate...differentiating between synaptic vs. extrasynaptic glutamate release. Building on the previous generation of the SF-...

  17. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...Meetings are for talking, not reading: Send around relevant documents, like proposals to review or project...discussions can happen “off-line” with just one or two relevant people. How often do you need to meet? Use trial...

  18. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...process had just been discovered, the mechanism was barely understood.” Doench’s graduate work helped expand...regulatory process. This early research is still relevant to his work at the Broad Institute today, where...

  19. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...lecture at iBiology. Commonly used filter sets & relevant fluorescent proteins Commonly used filter sets...

  20. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...bacteria, plasmids used for stable transfections rarely contain an ORI since the integrated DNA will be... Stably transfected cells may be considered an entirely new cell line from that of the original parental...
Showing: 321 - 340 of 617 results