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TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....no longer just for those working in academia or industry. At BosLab, we identify ourselves as part of a...creations that may not be pursued in an academic or industry lab. Mrina Nikrad, Ph.D., is an expert in microbial...
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3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....Physiology. Her research focuses on the neural circuitry involved in mechanical allodynia, a condition ... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m.... acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005...Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m.... anyway, including the entire faculty of the biochemistry department at the University of Massachusetts...changed since the injunction”. Postdocs around the country have now begun to speak up on this issue. These... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m.... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....ligated into a vector containing those overhangs. Entry DNA overhangs may be present in the original plasmid... 30-minute reaction. The destination vector and entry vector(s) are placed in a single tube containing... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m.... “cited by” and references sections of a PubMed entry are great places to look for more articles on your...contain your search term. When you view a paper’s entry, the number of citations that paper has is displayed... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m.... toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei Shi ...human cell lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....meetings of experts and leaders from academia, industry, community, education and government; advising...academic venues such as scientific, educational or industry conferences, as well as schools, community events... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....transmits retrogradely to first order neurons, via entry mediated by the G protein/G-protein receptor. Once...powerful tool that can be used to reveal brain circuitry, and continue to contribute answers to the complex... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....identifying PPIs, such as affinity purification mass spectrometry (AP-MS), lack the ability to specifically identify... purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec analysis... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....also culminated in a joint American Society for Biochemistry and Molecular Biology/ FoR Hack Day during which...FLSA) which mandates that all workers across the country either be paid more than $47,476 or be eligible... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m..... 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling in... into thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken, and... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...