... chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange,...Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...chromoprotein Ultramarine with hydrophilic amino acids to reduce non-specific interactions with cytosolic...research Read our Molecular Biology Reference Find plasmids for FRET References Blanquet RS, Phelan MA (1987...

...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and CRISPR resources...

...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...

...is because organic solvents can be quite harsh - lipids and soluble proteins can be lost during fixation... others. That said, organic solvents’ impact on lipids means that they are able to permeabilize your sample...examples of non-ionic detergents that interact with lipids and proteins in membranes and permeabilize non-...Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant antibodies at Addgene   ...

...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...

... the limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...glycines, serines and other small, non-aliphatic amino acids. However, long linkers increase the chance that ...fusions, our experience is that a linker of 4 amino acids (PVAT, when we use the AgeI site in the multiple...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...

...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...

...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the...on Addgene.org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan...

...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the... the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016/j.omtn.2017.06.011 Korneyenkov...AAV-Mediated Gene Transfer. Molecular Therapy - Nucleic Acids, 8, 13–25. https://doi.org/10.1016/j.omtn.2017.03.009...

...the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source...useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please ...mRNA can boost efficiency over transfecting with plasmids in some cell lines, most likely due to DNA toxicity...

...to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While this strategy greatly...Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential....2020.618020 Additional resources on the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When... ready to use AAV vectors at Addgene. Find AAV plasmids to use in your experiments. ...

...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr...

...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged... protein of interest or using non-natural amino acids to directly incorporate a fluorophore in your protein...self-labeling tag systems Tag Size (amino acids) Ligand Commercial ligands available Ease ...Various Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications...

...detect various targets, such as proteins, nucleic acids, or glycans. Fluorophores can also be covalently...should have high affinity and specificity to nucleic acids, with minimal background staining. Additionally,...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...More resources from Addgene Fluorescent protein plasmids and resourcesA practical approach to choosing ...

...Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology Plasmids Browse our CRISPR Guide Pages ...our CRISPR featured topic page Check out our Hot Plasmids Podcast Listen to our interview with synthetic...

...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors...to check out the Trono Lab website. If you have plasmids you’d like to deposit with Addgene, be sure to...

...other scientists save resources by sharing plasmids Find plasmids at Addgene ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...

...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on...Resources on the Addgene Website Find Cepko Lab Plasmids at Addgene ...

...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...

...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration...Addgene.org Read the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection...