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We narrowed to 582 results for: BASE;

Showing: 341 - 360 of 582 results
  1. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...team creates stabs for each plasmid being shipped based on the information in our lab software, labeling...
  2. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...to understand. Classes are based on the main Cas effector; types are based on main function and composition...composition; subtypes and variants are typically based on species of origin. There are currently two classes...
  3. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...a quick chat can be rather tricky (think people based in different countries). Here social media is a ...References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction...
  4. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...nucleotide level but share an overall structure of 13 base pair palindromic repeats separated by an 8 bp asymmetric...2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20. PubMed...
  5. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...results (Sinnett et al., 2017; Powers et al., 2019). Based on the work of the consortium, two gene therapy ...guide RNA and a hyperactive version of the human RNA base editing enzyme (“Editase”), ADAR2, into the hippocampus...iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References: Amir ...
  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...point on the axis determined by the researcher, based on their controls. You can usually tell where this...Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to...
  7. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single origin of replication...into a “pseudoknot” held together by intramolecular base pairs for the protein to be translated. An antisense...
  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton...immunogenicity typically associated with viral vector-based therapies. REVeRT supports advanced gene expression...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering...
  9. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...environment. The “how many?” question lies at the base of all these questions as there is not particularly...for their buck - make them demand more services based on what they're spending. Increase the number of...
  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...off-target effect. The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of...express without the G. Because the G is only one base, the Zhang lab usually adds it when they order the...oligo from IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could...
  11. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors (TALEs...
  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...popular counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." ...
  13. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...Since the number of base pairs for each varies, it is difficult to calculate this based on DNA concentration...
Showing: 341 - 360 of 582 results