We narrowed to 614 results for: CaS

...World Health Organization says the number of new cases could rise to 10,000 a week within two months. ...has been fatal in 70 percent of the most recent cases, primarily in West African countries. Even that ...

...the audio engineer and producer for the Addgene Podcast.     Additional Resources from Addgene Learn about... podcast...

...technology of the year in our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of.... So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,000 labs around the world...

...injection site and choosing a specific serotype, but occasionally you may want to further control where your gene...mouse line that contains a Cre-dependent mCherry cassette under the control of an otherwise ubiquitous promoter...

...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...

...Central (also eLife, PLOS and Figshare). Some use cases include: Cataloging a reagent’s performance as ...published, Zika researcher Christa Osuna collated Zika case studies, making a meta analysis available to the...

...positively affect scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed... helped accelerate scientific discoveries.” Occasionally, a plasmid tool is so influential that it is...

...genes at the site of insertion. In both of these cases, transposons combine the advantages of viruses and...2) a transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping...

... the other posts in this series. Listen to our podcast interview with Connie Cepko to learn about her ...leaders rarely give orders (although they must occasionally). Good leaders exert influence in other ways...

... a PI who is a good mentor is important in both cases. As a PhD student you need to learn how to be a ...you pursue any career alternative? Listen to our podcast interview with Harvard Medical School researcher...

...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...

...published a description of the VGS process and use cases in Human Gene Therapy. Existing methods for AAV ...vector genomes as containing just the expression cassette that we want, it also includes a small amount ...

...transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by...CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: ...

...non-specific staining can be a challenge in the best of cases. But it can be particularly challenging when your...primary antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another...

... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...protein functionality (Arribere, et al). In rare cases, tags can also affect protein localization and solubility...

...of a hairpin structure within your primer. In our case, we will add TAAGCA, resulting in a final Forward...to the sequence to be amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying...

... strategic knowledge to solve a clinical disease case. As a teacher, you explain the game rules and then...face each other on a single chosen clinical disease case. Then, each team received a whole set of cards (...

...really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells,... delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are...

... Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs...technologies are too time consuming in the first case and nonspecific in the second. Fatima therefore ...

... common event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General...28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV vector as...