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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 709 results for: LIC

Showing: 341 - 360 of 709 results

  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...as they do science outreach and engage with the public, and learn from the process of writing papers and...broader impacts for their work in education and policy and to draw attention and support to work in their...before you complete your work. If you’re giving a public talk, ask a friend who works in a different field...

  3. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

  4. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...antibody and content team, we identified what applications should be included and what basic information...through an editing process and final check before publication.   Do research! Nearly everything we write entails...make figures in BioRender or use appropriately licensed and cited figures from papers or image sharing...

  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...their reagents with the scientific community.   (click to enlarge) As we look ahead to the things to come...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...neuroscience method that lets you fire neurons with the flick of a light switch. Neurons are not typically persuaded... expression in cultured neurons and mouse brain slices collected following intracranial injection of an... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...of DNA that can potentially complicate results of a downstream application such as quantitative PCR to...is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-... that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID can also be used...

  10. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...methionine. The labeled proteins can be detected using click-chemistry that covalently links the azido containing... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement...self-process pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  12. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...and in the intersection between science and public policy. Follow David on Twitter @driglar.     References...professor of economics at the Andrew Young School of Policy Studies at Georgia State University, is an expert...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...the body through the bloodstream, and eventually elicit their actions on other tissues. At the Nuclear ...a broad range of reproductive, developmental, metabolic, and immune response programs. NRs also encompass...gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators Coregulators constitute...

  14. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ... when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked by dCas9....for dCas9 to interfere with DNMT1 even through replication. The dCas9 also needs to be removeable, otherwise...doi.org/10.1038/s41467-021-25991-9. Spruijt CG, Gnerlich F, Smits AH et al. (2013) Dynamic readers for ...

  15. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ... reduced off-target editing frequencies. For applications that require high DNA editing specificity, for...deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing...editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038...

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...genotype and phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You...imagine, there is an array of distinct goals within metabolic engineering, synthetic product production, and...

  17. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...originally described in a peer-reviewed scientific publication. We love science, but we don’t have the resources...be fairly fast, getting the full answer to a complicated question may take some time. If the customer ...message Answering customer service questions is a delicate balance of knowledge, patience, and diplomacy,...

  18. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...hypomethylation, on the other hand, has also been implicated in the development and progression of cancer ...challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based biosensor...:12404098.  4. Lin, Chi-Wang, Cindy Y. Jao, and Alice Y. Ting. "Genetically encoded fluorescent reporters...

  19. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...recently there has been a counter movement by the public to access basic equipment and to become involved...first hackerspace which now offers members and the public cheap access to personal manufacturing tools – ...woodworking tools, and a metalshop. After two summers in Silicon Valley as a student and then instructor at Singularity...

  20. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... and limited the exchange of information to the public and to other academic labs. Being unable to publish... organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry ...something new and beautiful, to engage with the public in a meaningful way about science, and to have ...
Showing: 341 - 360 of 709 results