We narrowed to 616 results for: LIS
-
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....FLiPs) Fluorescent proteins can be applied in a long list of creative ways. In her graduate work in Michael... lab at Stanford, Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to...
-
Trading Spaces - Visiting Scientist Edition
TypeBlog PostPublishedAug. 22, 2023, 1 p.m....researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...will need, any required training, and a priority list of experiments you hope to complete. Share this ... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....enzymes, the app is quite helpful and provides a list of directions including which buffers to use and... I have found myself wishing that it had a codon list for each amino acid, but, overall AMINO can be beneficial... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....further introduce him to the Addgene community. Listen to the interview here! Doench majored in history... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed that many other...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m.... from this collection in your experiments and published your data, please fill out this form and we’ll...complete QC and you can find all available plasmids listed on Ginkgo’s collection page. Bacterial expression... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....gene of poorly classified function and a well established cellular signaling gene, thus providing a starting...publications, we used the cancer plasmid collections listed above to demonstrate how gene expression profiling... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of an antibody isotype...cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting. The five isotypes of antibodies... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which...on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...community to fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....Finally, as previously mentioned, enlist the help of your network to polish the final draft prior to submission... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....plasmid carrying the required gene. The table below lists some of the most commonly used selection markers...said element. This link provides a more extensive list of yeast auxotrophic markers and includes the associated... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical School... the internet, but start by downloading my handy list of suggestions. Get started. Have regular meetings... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....BASU, is derived from a BirA* homolog found in B. subtilis. BASU was compared head-to-head with the E. coli...IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, Ramanathan... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....incorrect; take it instead as valuable feedback. Listen. Think about the questions you’ve received, read...read the comments, seek feedback from others, and listen quietly as people discuss their experiences. If... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....affinity reagents. Types of Affinity Reagents (Listed in no particular order.) Fab fragments are created...distinction of being the only affinity reagent on this list derived from nature. Single-domain antibodies are... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...