We narrowed to 619 results for: LIS

...FLiPs) Fluorescent proteins can be applied in a long list of creative ways. In her graduate work in Michael... lab at Stanford, Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to...

...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...will need, any required training, and a priority list of experiments you hope to complete. Share this ...

...enzymes, the app is quite helpful and provides a list of directions including which buffers to use and... I have found myself wishing that it had a codon list for each amino acid, but, overall AMINO can be beneficial...

...further introduce him to the Addgene community. Listen to the interview here! Doench majored in history... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many...

... from this collection in your experiments and published your data, please fill out this form and we’ll...complete QC and you can find all available plasmids listed on Ginkgo’s collection page.  Bacterial expression...

...monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of an antibody isotype...cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting.  The five isotypes of antibodies...

...lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed that many other...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...

...gene of poorly classified function and a well established cellular signaling gene, thus providing a starting...publications, we used the cancer plasmid collections listed above to demonstrate how gene expression profiling...

...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...community to fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine...

...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which...on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure...

...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...

...Finally, as previously mentioned, enlist the help of your network to polish the final draft prior to submission...

...plasmid carrying the required gene.  The table below lists some of the most commonly used selection markers...said element. This link provides a more extensive list of yeast auxotrophic markers and includes the associated...

...group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical School... the internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings...

...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

...incorrect; take it instead as valuable feedback.  Listen. Think about the questions you’ve received, read...read the comments, seek feedback from others, and listen quietly as people discuss their experiences. If...

...affinity reagents.    Types of Affinity Reagents  (Listed in no particular order.) Fab fragments are created...distinction of being the only affinity reagent on this list derived from nature. Single-domain antibodies are...

...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...