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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 711 results for: Met

Showing: 341 - 360 of 711 results

  1. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...research. Long story short, telling a good story is something that can be learned, and will serve you well in...yourself jumping backwards in time to explain something, rethink your structure. Develop your protagonist...but there are plenty of other options. Choose something with emotional significance that is likely to ...

  2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...viral production strategies or developing novel methods for data analysis, our team’s goal is to provide...and laboratories alike as an easy and inexpensive method to improve AAV yields. Although we only investigated...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

  4. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...live audiences in your future.     3. Start something In almost every career talk I present, I’m asked...before others do”. Candidates who have “started something” which they can share on their resumes have a ...oreal USA For Women In Science If you have a method that you've been working on, let us know in the...

  5. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...First, it should “hook” your audience in with something that sparks their interest and makes them want...bring you and your audience - via the scientific method - to the resolution, or the ending of the story...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...

  6. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that ...tags are frequently used for western blot, flow cytometry, and immunofluorescence . However, protein purification...tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t...

  7. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...the pocket of a folded protein, or blocked by something bound to the protein, the antibody can't bind ...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...

  8. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...transfected with three different vectors and flow cytometry is used to sort populations by mCherry and GFP... base-editing efficiency using XMAS-TREE-based methods at various genomic loci in four different categories...Cas13Y, two compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered...

  9. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...Experimental design & methods: These questions are focused on injection and detection methods. For example, you...until performing the experiment? What detection methods did you use for expression of AAV cargo? Did you...

  10. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...light. In a recent article published in Nature Methods, Boyden and his team describe the discovery of ... tree of life. Our most recent paper in Nature Methods offers the first true two-color pair. We'll be ...Neural Populations. Nathan Klapoetke et al. Nature Methods. 2014 Feb 9. (PubMed). Read More: Other Optogenetics...

  11. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples on dry...following transport or storage on dry ice. Nat Methods. 2013 Apr;10(4):278-9. PubMed PMID: 23538862. 2...recombinant adeno-associated virus vectors. Mol Ther Methods Clin Dev. 2014 Aug 13;1:14034. PubMed PMID: 26015974...

  12. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...this Plasmid” section. Having IDs linked to these metadata and publications help to enable reproducibility...cite any Addgene plasmid in your materials and methods section have been updated to include the option...how to cite using the PIDs in the material and methods section of your publication. You should carefully...

  13. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ... biosensors, which are optimized for specific parameters: jGCaMP7s is the most sensitive responder to...primary inhibitory neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal...Optogenetics Optogenetics continues to be a useful method for manipulating specific neurons. We recently ...

  14. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... more effective mAb therapeutics. The in silico method of immunogenicity assessment can help in determining...All approaches have their own limitations. Using methods together can inform decisions about in vivo application...for Sequences Containing Unnatural Amino Acids: A Method Using Existing in silico Algorithm Infrastructure...

  15. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...rate that study as untrustworthy, inaccurate, methodologically flawed, and biased. As a result, the control...because a greater familiarity with scientific methodology makes it easier to spot even minor weakness or...might struggle to understand. Why should we trust something that we cannot read or verify for ourselves? Further...

  16. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...exported as a picture. I believe that showing something different from time to time breaks up the monologue...monologue a little and gives participants something else to focus on. Use collaborative cloud apps It is... Take brain breaks Meetings can get very long sometimes, and interaction fades away the longer we are ...

  17. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...support your favorite local exercise spot or find something new. We usually have yoga class twice a week at...more independently. For example, they could do something like puzzling or crafts, while listening to podcasts...registered report, where peer review is based on the methods, not the results. Also consider using this framework...

  18. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision. Our...or double stranded donor DNA with other design parameters. Example single stranded donor DNA and a presumptive...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...probe and build new systems. Such goals require methods to directly activate and inactivate biological ...that prints a chemical image, as well as a genetic method for the computational problem of edge detection...identified in bacteria, and with similar optimization methods, many of these systems could be made suitable for...

  20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing ... al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated...
Showing: 341 - 360 of 711 results