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We narrowed to 849 results for: NTS

Showing: 341 - 360 of 849 results

  1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...base editing events in real-time but also enrich for base-edited cells in their experiments. In the past...TLDR: The eGFP reporters from the Harris Lab circumvents the need for DSBs in monitoring on-target DNA...reporter that detects and enriches for base editing events in vivo without relying solely on GFP (Katti et...adaptable tool to identify and enrich for base-editing events in vivo. TLDR: Gene On (GO) from the Dow lab is...is a flexible reporter system for base-editing events in vivo. As GO relies on the correction of a mutated...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...diagnostics, genotyping, and biotechnological advancements. Many methods of nucleic acid detection however...presence of SARS-CoV-2 RNA, but inadequate access to reagents and equipment has become a bottleneck. In the..., scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple, portable...for SHERLOCK version 2. Here are some of the improvements: SHERLOCKv2 uses far less primer in the pre-amplification...low-frequency cancer mutations from cell-free DNA fragments as well as health-related single nucleotide polymorphisms...using a dipstick in under one hour. While the lab points out that the protocol is not approved for clinical...

  3. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...codons encode single amino acids. Evolutionary constraints have molded which codons are used preferentially...in any organism. So what are the evolutionary constraints that lead to these preferences and what can we...2005). How does codon usage bias affect my experiments? While codon preferences can be very useful for...production of nonfunctional proteins and protein fragments respectively. Solving the problem of codon usage...your expression host before using them in your experiments. Expression of alternative tRNAs If you don’t...producing alternative tRNAs may not express sufficient amounts of your protein of interest. Regardless of which...

  4. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...give general experimental guidance, and resolve complaints about products. As you might expect, different...necessary. The job description and the candidate requirements (and maybe also the proposed salary range) should...lab, to travel to customers’ sites, and to run experiments with/for them. Though not the most prestigious...support roles also involve interacting with other departments such as research and manufacturing, quality assurance...structure you may be able to move into one of these departments from technical support. Technical support careers... is particularly excited to hear about novel developments in the fields of gene and cellular therapies...

  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...quite successfully adapted for use in screening experiments as well). Therefore, if you’ve started down the...target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately...placed in multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression...have your F0 generation (barring life’s little accidents and stuff ups). This is a huge improvement on ...

  6. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...samples, and treatments may affect the assay. It’s unusual to need to adjust amounts of reagents in your chemistry...and your reaction optimized. You’ll want to run gradients for optimal annealing temperature and optimal ...can interfere with amplification reactions, and reagents can be used up quickly by abundant transcripts...abundant transcripts. However, adding too many reagents can prevent your reaction from reaching the plateau...for publication of quantitative real-time PCR experiments. Clin Chem. 2009 Apr;55(4):611-22. doi: 10.1373...

  7. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...have shown recently that desired genome-editing events by HDR can be generated more efficiently by (1)...as APP and PSEN1, in most cases the intended HDR events were corrupted by unwanted indels on the same allele...correctly edited alleles, when screening reasonable amounts of single cell clones. Furthermore, CRISPR/Cas9...controlled over-active Cas9 and repeated our experiments with blocking mutations, we still weren’t entirely...Alzheimer mutations we study are heterozygous in patients. As the probability of mutation incorporation ...the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...for synaptic glutamate response. They generated variants optimized for different applications (v82 has ... and powerful high-throughput screen to select variants based on performance metrics specifically for ...Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living...BeadScan platform for screening candidate biosensor variants for performance under two-photon fluorescence ...temperature indicator B-gTEMP designed to meet those requirements (Lu et al. 2022). By fusing two bright fluorescent...about your favorite new biosensor plasmids in the comments below!   Additional resources on the Addgene ...

  9. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...and recombination events). TIDER can screen for specific homology-directed repair events and small edits...significant number of cells have been edited. For many experiments, the end goal is to generate a clonal cell line... Fig. 2: Size screening for large deletion events.   Validating knock-in mutations You’ve introduced...combo and are hoping for homology-directed repair events, or maybe you’re performing a single base pair ... flow sorting or drug selection, assuming your reagents are amenable to this. If delivery isn’t an issue...

  10. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...solutions are discussed below. There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP...applications that require anaerobic conditions. In experiments requiring anaerobic conditions, proteins that...Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under acidic conditions, with...genetically encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date...is used to excite GFP, may also excite several components that are naturally present in cells or media....although it still is worthwhile to generate several variants and check the functionality of the fusion (Mastop...

  11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... can use AAVs to improve your genome editing experiments (with and without targeted nucleases). AAV genome...identified by PCR. Then, if necessary for subsequent experiments, the floxed neoR gene is removed via infection...researchers have devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied...2017 recently showed that by delivering CRISPR components as RNPs and delivering two halves of a repair...possible sequentially repair a target site with the contents of the two AAVs. Using this technique, Bok and...can provide a powerful boost to genome editing experiments. We’re excited to see how you continue to use...

  12. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ... question you think rotation students should ask senior graduate students? 11:51 - 13:28 : How do you ..., we sit down to talk with two senior graduate students, Ben Vincent and Niroshi Senaratne, from the Biological...down of graduate student life. Senior graduate students can sometimes be elusive or have a certain mystique...program. Don’t be fooled! Most senior graduate students are friendly and full of useful advice. Stay tuned...

  13. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...and is useful for direct imaging of events in cells and brain explants. This construct is available through...Nano-Lanterns including monitoring multiple cellular events such as the dynamics of subcellular structures ...NanoLuc® luciferase Many intracellular sensors of events like calcium release have been made using fluorescent...PMC4942401. 5. Susuki, K., et al. (2016) Five colour variants of bright luminescent protein for real-time multicolour... more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein Biosensors...

  14. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...charge). Larger fragments of linearized DNA migrate slower than smaller linearized fragments. You can separate...have a full plasmid ready to be used in your experiments.     Alternatively, this whole process can be...sell fast digest enzymes that can digest large amounts of DNA in as little as 10 minutes, but check with...You then add DNA ligase to covalently link the fragments together at the expense of ATP (see below, covalent...Find the Perfect Empty Vector for Your Cloning Experiments Browse Our Molecular Biology Resources ...

  15. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...funding for research, and also to encourage women, students, and underrepresented minorities to study STEM...them share their lab experiences, findings, and experiments as articles written for general audiences. So...scientists and doctors with their questions and comments.  Lastly, we are planning to launch an e-magazine...Instagram every Sunday. Undergraduate and graduate students are more than welcome in contributing to The Addictive...scientists. Keep your message to the main three points of your story. Interested in learning more? Visit...

  16. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ... more transparent. Second, SITN helps graduate students develop their communication skills and engage ...to a layperson. They also enjoyed mingling with students and other attendees and getting the chance to .... The future of DayCon DayCon and other similar events show how meaningful science outreach can be, both...both for scientists and the public. For students, these opportunities to improve our communication and...efforts to engage the public - let us know in the comments section! More information about SITN: DayCon...

  17. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ..., we heard over and over again that the legal documents were one of the biggest hurdles for scientific...documentation that allows you to conduct your experiments with confidence. Benefits of the UBMTA: Peace...Even with all those benefits, we know that legal documents can get tricky. Here’s how Addgene helps: For ...materials have been requested and by whom. For Recipients: Streamlined Processing: When you place an ...So go ahead and use Addgene plasmids in your experiments. You know that they’re properly covered for when...

  18. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...communicators. In this chapter we learn about some of their events - science seminars, science cafes, and the SITN...discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs SITN runs ...scientists from a variety of fields as well as students from the ed school, design school, and Kennedy...short-form blog posts help readers think through current events. Chapter 6: The SITN Editorial Process -Vini discusses...balances working in the lab and working with SITN. She points to collaboration and delegating as keys achieving...

  19. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their ...their behavior. These ants become “zombies” and leave their foraging trail. They climb upwards until they ...temperature and humidity favors fungal growth. The ants bites down and locks their jaws into a leaf, and... two cells. At the same time, it sucks out its contents (hence the name Vampirovibrio). What’s left of...

  20. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... CRISPR/Cas9" from the Journal of Visualized Experiments (JOVE). The video publication by Stuart Orkin...produce intrachromosomal deletions based on the experiments of Kim and colleagues using zinc finger nucleases...The first is the study of non-coding regulatory elements. Regulatory DNA function is typically inferred...exploring the function of non-coding regulatory elements in their native chromatin context. An alternate...paired DSBs is inversions [3]. These rearranged segments may also be informative as to genomic function...
Showing: 341 - 360 of 849 results