We narrowed to 488 results for: RET

1. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will...

2. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...

3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These...

4. Antibodies 101: Isotypes

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   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

5. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

6. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

7. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

8. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

9. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

10. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ...

12. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...transgene is flanked by two ITRs, and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector...

13. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ..., does not require standard curves, and can theoretically detect a single target molecule in a sample....

14. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

16. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

17. To Each HIS Own

    Type

    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ... specific proteins within a system. This can be pretty complicated given that a single cell has tens of...

18. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

19. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

20. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...