We narrowed to 484 results for: RET

1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These...

2. Plasmids 101: Screens vs. Selections

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   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

3. Hot Plasmids - May 2022

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   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

4. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

5. Antibodies 101: Isotypes

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   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ...

7. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will...

8. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

9. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

10. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

11. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

12. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

14. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

15. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the...

16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

17. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

18. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...

19. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...

20. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going...