We narrowed to 464 results for: SHI

1. Addgene's Top 10 Blog Posts of 2015

   Type

   Blog Post

   Published

   Dec. 7, 2015, 6 p.m.

   ... we’ve experienced incredible growth with our readership expanding from ~10,000 users per month in November...

2. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

   Type

   Blog Post

   Published

   April 7, 2015, 4:21 p.m.

   ...heterologous genes, but the trend of research has shifted in recent years toward tinkering with regulatory...

3. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   Published

   April 4, 2023, 1 p.m.

   ...an error-prone pathway frequently introducing frameshift knockout mutations. Thus, if genetic manipulation...

4. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ... in addition to preparing DNA and generating/replenishing kits, is to find new ways to make lab processes...

5. Addgene Depositors Get More Citations

   Type

   Blog Post

   Published

   April 20, 2017, 2:30 p.m.

   ...citations in its first four years (1). In addition to publishing in Science, Professor Zhang deposited the associated...

6. Science Career Options

   Type

   Blog Post

   Published

   Feb. 23, 2017, 1:30 p.m.

   ... then decide on a course of action. Instead of rushing in, you take steps to make it more likely you’ll...

7. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   Published

   March 14, 2019, 1:54 p.m.

   ..., et al. "Naturally enveloped AAV vectors for shielding neutralizing antibodies and robust gene delivery...

8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

   Type

   Blog Post

   Published

   Dec. 20, 2018, 1:15 p.m.

   ...Charlotte, all of whom are still working on theirpenmanship. Additional resources on the Addgene blog ...

9. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   Published

   Sept. 19, 2017, 1:20 p.m.

   ...by the dL5** FAP.  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is...

10. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    Published

    July 21, 2017, 12:48 p.m.

    ...thanks to our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has...

11. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ... in Douglas Fowler's lab at the University of Washington sought to address the effects of genetic variants...

12. 5 Great Apps for Lab Life

    Type

    Blog Post

    Published

    Sept. 22, 2016, 2:30 p.m.

    ...selenocysteine and pyrrolysine. I have found myself wishing that it had a codon list for each amino acid, but...

13. Resume Writing for Non-academic Science Careers

    Type

    Blog Post

    Published

    Dec. 18, 2014, 4:06 p.m.

    ... grant application? Are you familiar with the publishing or patent application processes? How about facilitating...

14. Advice for Scientists Starting a Lab

    Type

    Blog Post

    Published

    April 29, 2014, 1:43 p.m.

    ... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

15. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...-Cas9 tools have been engineered to introduce frameshift mutations in genes of interest, turning expression...

16. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...alongside your input control. Elute the DNA after washing and then incubate with Proteinase K to reverse ...

17. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi Kimura’s labs have recently published a set of ...

18. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ... inserted or deleted, can result in an in-frame shift of mCherry and thus expression. As a proof of concept...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove...