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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 581 results for: SIM

Showing: 341 - 360 of 581 results

  2. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter...

  3. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar...because they had close relatives suffering from similar diseases. Others were inspired by the imminent ...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...Mismatch Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing...

  5. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...discussed below. The software could also be used as a simple tool to rapidly find one or a few gRNAs suggested...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

  7. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...CD45 expression turns out to be weak. Table 2: A simple flow panel to detect live, transduced, and CD45...reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...://doi.org/10.1371/journal.pone.0016765. Moore, Simon J., et al. “EcoFlex: A Multifunctional MoClo Kit...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons where...Allen Mouse Brain Connectivity Atlas website. A similar approach was used by researchers from the Janelia...

  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ..., add the various reagents to a single tube and simply flick the tube several times. This will help ensure...

  15. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who wrote a guide to selecting...

  17. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ... me best?     But between these options, we simply can't decide! Abi, Lin, or Blottie? Which name ...

  20. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...snowflakes, since snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab...
Showing: 341 - 360 of 581 results