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Showing: 341 - 360 of 602 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...conferences, but after receiving her degree she was faced with a similar conundrum – she had the skills, training...color changing flower project was inspired by a Facebook comment.  In our day job, we worked on plants ...

  2. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...so many technologies currently available, you now face the challenge of trying to figure out what system...Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits! Efficiency: Wouldn...

  3. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...comfortable with awkward silences but I’ve learned to embrace them after one ended with the person in front of...your manager for advice if you’re not sure how to face a particular situation. But keep in mind that a ...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ..., but those outside of your field can point out places where you gloss over essential details or omit ...avoid rushing out the door without your poster, place it by your keys a day in advance. Dress for the ...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...applications across a wide range of topics. He embraces open science, self-publishing directly on his .... R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore...

  7. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...simply as it seems laid out above, there are many places where the PhD application cycle becomes much more...program will allow for you to have the time and space to delve into what matters to you. It can also be...

  10. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...track protein localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally...overexpressed fluorescent protein fusions is to replace the genomic copy of your gene of interest with ...

  11. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

  13. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers need...between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens ...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...individual plasmid orders, because when a PI or lab places an order, they are likely to order several plasmids...next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... journal club? Where will the journal club take place? Preparing for the first meeting There are many ...important pieces before that first meeting takes place to ensure success. One of the most important is ...

  16. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...required for a mini amplification reaction to take place.  When the PCR is complete, a droplet reader extracts...contamination. Optimize your PCR For a good starting place on what PCR parameters to use, see Lock’s seminal...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...labeling so that a cell’s projections are easy to trace and not lost in a jumble of fluorescent neurons.... But notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green...

  18. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...samples accurately and in a way that can be easily traced. If you’re just starting a lab, or are looking ...barcode can store a lot of information in a small space and is ideal for tracking large collections. Many...
Showing: 341 - 360 of 602 results