...signal at a point on the axis determined by the researcher, based on their controls. You can usually tell...intimated if you’re struggling to understand how the researchers decided to place their gates on the plots! Flow...gating strategy, you can start to ask why the researchers made the decisions they did in their experiments...vitro chondrocytic differentiation: A stem cell research initiative at the King Abdulaziz University, Kingdom...

...pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled... pooled libraries and one of our most popular barcoding libraries as pre-made viral preps. If you can’...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...feel more confident incorporating them into your research. And, of course, if you have any questions, don...

...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...

...Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance their production either in the plant...help in screening candidate genes and speeding up research. Certainly, there is still more to transient leaf...your lab, it can become a strong tool for your research. Feel free to add further tips and experiences... in the Lee-Parsons Lab.     References D'Aoust, Marc-André, et al. "Transient expression of antibodies...

...the College of Life Sciences that same year. Her research is focused on the escape mechanism of genome editing...Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-encoded...professor in Synthetic Biology in 2006. The Yang lab's research is focused on developing microbial genome editing...Sciences in September 2019 as a Ph.D. candidate. Her research focuses mainly on the development and application...

...and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124...cleavage by Cas9 in living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332...enrichment (TREE) in human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713...the CRISPR guide Find CRISPR plasmids for your research ...

...users, we just released a major redesign of how search works on our site. For Addgenies, development includes...with our software. When this happens, we need to research and hopefully find a comparable library if it’...our users focus on the fun stuff, accelerating research and discovery. We work with transparency, gladly...the Addgene blog Learn about what went into our search engine update Find all Addgene news Resources ...

...in neuronal circuits or synaptic remodeling, researchers combine the Cre and FLP recombinases to control...combination of Cre, FLP, and inducible systems gives a researcher exquisite control over expression of multiple...Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how...-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids...

...understanding the brain’s function is knowing its architecture, in particular the connections between different...to their advantageous characteristics for brain research and their ability to introduce genetic material...fluorescent proteins (red, green, blue). If a researcher injects a cocktail of these different AAV vectors...Atlas website. A similar approach was used by researchers from the Janelia Campus for their MouseLight ...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2...

...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite Cas enzyme...generally low; to increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate...repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand...

...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if your...this file over the years and a talk about your research or work will write itself when the time comes....ask about how many people use plasmids in their research and then see how many of them have requested from...

... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... variety of ways to manage my time. As a busy researcher, you’ve probably already started coming up with...hope you’ll find this post useful. After a bit of research and thinking about my own processes, I’ve broken...

...variety of organisms.  While much of current CRISPR research focuses on its potential applications for human...engineering tools that are available to academic researchers through Addgene. CRISPR components for plant ...promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos with minimal cloning... hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing...

...blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to ...combination with retinal) variations designed to give researchers flexibility in using optogenetic tools in their...concentration gradient, and of activation inhibitory (ArCH) proton pump passing ions against the concentration...proteins activate their signaling pathways allowing researchers greater control over when and where to activate...

...increasing affordability and accessibility, more researchers have been utilizing full plasmid sequencing services...sequencing that Addgene uses.) However, when some researchers received the sequencing results for their plasmids...numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid features that ...monomeric plasmid compared to DH5α. However, more research is needed to determine which recA- strains are...

...135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez J (2010) DNA Transposons...different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping...encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to cells or a ...

... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic...

...effectiveness and interpretation of RNAi. Fortunately, researchers studying plants and other organisms lacking this...concatenated. hpRNA has been extensively used in plant research and many different constructs are available to...and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for shRNA in...fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA and visual screening method...

...how and where you pursue it. It can manifest via research and data gathering, regulatory efforts, policy...fellowships have a strong focus on early career researchers who have decided to transition out of academia... host “Hill Days” where they train and bring researchers to Congress to advocate for increased science...incentivizing the real-world impacts of health services research. She is particularly interested in driving scientific...

...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038/s41592-021-01338-6 Grimm...