We narrowed to 700 results for: eed

...plasmid compared to DH5α. However, more research is needed to determine which recA- strains are most effective...2009), pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple...production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol...

... researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative...end-joining activity, and for others, there is a need for the exclusive ligation of fully base-paired ...amplification using cloned thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991...

...packaging capacity, AAV vectors were suitable for our needs for the following reasons: Safety: Since AAVs ...should be used to clean AAVs from workspaces. Speed: AAV transduction experiments are relatively quick...useful for a wide variety of experiments, care still needs to be taken when selecting which particular AAVs...

...DNA sequence encodes the proteins the transposon needs for transposition. Transposition protein: This is... transposons” since they encode the protein they need to move around, either a transposase or a reverse...transcriptase. Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase...

...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more... injury in the acutely inflamed glomerulus." Proceedings of the National Academy of Sciences (2016): 201606253...cell subsets in the skin-draining lymph node." Proceedings of the National Academy of Sciences 113.4 (2016...

...the end, this results in an experiment that could need more than 8x107 successfully targeted cells. Depending...the analysis becomes easier, the number of cells needed is lower, and perhaps most important, targeting...good idea to keep a bioinformatician close if you need help putting your list together. Once you have your...

...should also be considered. If the light intensity needed for activation is too high, the photoactivation...photoswitching between two fluorescence wavelengths. Indeed, within the world of super resolution microscopy...UV-inducible green-to-red fluorescence conversion." Proceedings of the National Academy of Sciences of the United...

...grow haywire, access and create more vasculature to feed themselves, use the blood stream to commute to and...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted... inhibit expression of co-stimulatory molecules needed for T cell effector function. Cancer cells also...

...as excitation wavelengths and emission profiles, need to be matched with the experimental setup and objectives...Resonance Energy Transfer (FRET) applications, the dyes need to have compatible excitation and emission profiles...witnessed remarkable advancements, driven by the need for more precise, stable, and versatile imaging ...

...we’ve included a glossary at the end if you ever need to double-check your vocabulary! A brief review ...double-strand breaks. The only things base editors really need from Cas9 are sequence specificity and the R-loop...efficient and precise editors. Most researchers agreed on the first part of the likely editing mechanism...

...would like you to meet...  Hmmm, they need a name. And we need your help in finding a good name for Blugene...

...should our new blog be any different? If you still need an extra push, there’s evidence that laughter really...’s a way to connect with other people. (We don't need evidence for that one, do we?) OK, so, now that ...

...   Or maybe your experiment went so well you need to express yourself. We have something for that ...celebrate those wins in the lab.   You don’t need to sweep confetti off the floor of your lab if you...

...though I suspect that our costume contests were indeed occurring earlier but that the photo evidence was...how seriously we take costume design. In case you need more convincing, I’ve got just the proof: Above...

...biologists alike. Now with over 600 articles, we needed a better way to organize and present the vast collection...outside of these five areas and you can find what you need by field (ex: microbiology, neuroscience), or explore...

...choosing the cargo, serotype, and prep size they need for their experiments — and we’ve been able to ship...continue to expand the collection to meet the diverse needs of our community and support a wide range of experimental...

...in interactive figures is a great step up, but I needed a way to show different aspects of the data simultaneously... R can further customize the dashboard to their needs. I hope you find the dashboard useful when analyzing...

...developing a strong graduate student community and the need for belonging in the scientific environment 3:09...graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post Grad Plans 17:07 - 18:29 ...

...take on the risk of developing products that are needed but that are high risk? 34:37-36:53 : Was It difficult...more important to develop something that could succeed in the market or to focus on a technology that ...

... commitment to be responsive to the community’s needs and our commitment to top notch quality service ...as we grow and change to meet its ever-evolving needs. For now, we have many exciting things planned ...