We narrowed to 533 results for: erf

1. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

   Type

   Blog Post

   Published

   Nov. 12, 2019, 2 p.m.

   ...IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie...

2. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   Published

   Oct. 10, 2019, 12:41 p.m.

   ...GFP in imaging  While GFP has been an immensely powerful tool to study proteins in vivo, it does have some...

3. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   Published

   Dec. 13, 2018, 1:14 p.m.

   ...viral service but did you know that Addgene also performs independent research? Since launching the viral...

4. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   Published

   June 27, 2018, 1:16 p.m.

   ...isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is even able to ...

5. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   Published

   Jan. 25, 2018, 2:09 p.m.

   ...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

6. Antibodies 101: Epitope Tags

   Type

   Blog Post

   Published

   May 11, 2023, 1:15 p.m.

   ...obvious con of GFP is its size – which can possibly interfere with protein function and localization. If you...

7. Using Addgene's New Viral Service for Your Research

   Type

   Blog Post

   Published

   Nov. 17, 2016, 3:30 p.m.

   ... gene, replace it, modulate its expression, or perform a genome-wide screen, we’ve got viruses to help...

8. Plasmid Cloning by PCR

   Type

   Blog Post

   Published

   March 29, 2016, 2:30 p.m.

   ...Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research ...

9. DAP arrays for multiplex gene editing

   Type

   Blog Post

   Published

   July 7, 2022, 1:15 p.m.

   ...tRNA stood out from their initial testing. It outperformed other tRNA candidates (either from human or ...

10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type

    Blog Post

    Published

    June 23, 2022, 1:15 p.m.

    ...limitation. The procedure will also need to be perfected until the efficiency of repair approaches 100 ...

11. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type

    Blog Post

    Published

    June 1, 2021, 1:15 p.m.

    ...laboratory (BSL-3), a high-security facility needed to perform the experiments to demonstrate that this approach...

12. Networking Follow-up: I Met a Person. Now What?

    Type

    Blog Post

    Published

    Jan. 12, 2021, 2:15 p.m.

    ...thank you isn’t necessary. I once got a box of 6 perfect, chocolate-covered strawberries for helping with...

13. Celebrate National Book Month with Addgene

    Type

    Blog Post

    Published

    Oct. 3, 2019, 12:47 p.m.

    ...Designer Sarum: The Novel of England by Edward Rutherfurd "A 10,000 year history of southern England. While...

14. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    Published

    Sept. 12, 2019, 1 p.m.

    ...the primary inhibitory neurotransmitter - is a powerful method for understanding how this molecule impacts...

15. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...a red fluorogenic protease reporter based on superfolder Cherry (sfCherry). Like FlipGFP, sfCherry was...

16. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...sgRNA-Sirius-8XPP7 and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow...

17. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type

    Blog Post

    Published

    Dec. 20, 2018, 1:15 p.m.

    ...CRISPR Babies is that the scientific process, as imperfect as it may be, does work when it is followed. He...

18. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...database is to browse or search information in more powerful ways, and the advanced search page simplifies ...

19. The MTA at Addgene

    Type

    Blog Post

    Published

    July 23, 2024, 2:17 p.m.

    ... what to expect. No system, including MTAs, is perfect, but it does provide consistency and transparency...

20. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...   Zebrafish experiments with MG-2I-dL5** were performed with embryos and adult fish. Zebrafish are an ...