We narrowed to 411 results for: genome
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TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent protein...
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Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....pyogenes Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...methods and tools for studying chromatin and the epigenome. If you have been considering some new techniques...Regulation CRISPR 101: Epigenetics and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA...Experiment with Enhancers and Epigenomics I would like to thank the many scientists with me at the Keystone ... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....Addgene! Some bacterial strains contain lacZ in their genome, and scientists have found that when a portion ... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....are captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40% layer... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....pathway for the ncAA integrated into the cell’s genome, instead of incorporation via plasmid or media.... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....thousands of plasmids, using both mass production genome wide screens and the more traditional “one at a... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....2017) Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . ... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....transformation acceptor vectors, and sequences for genomic integration by homology directed repair. Using ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m.... for your synthetic biology experiment Read our Genome Engineering Guide ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....barcodes that are integrated into target cells' genomes upon infection. When using the ClonTracer system... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....receptors and signaling cascades conceivably allows the genome to sense the impact of the total environment on... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....have to be minimum around 1012-1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....in years past, we've been working with the Rare Genomics Institute to provide plasmid grants to researchers... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying which sgRNAs... FIRE-Cas9 for inducible interrogation of the epigenome Article contributed by Joanne Kamens Listen to...new system, Fkbp/Frb Inducible Recruitment for Epigenome editing by Cas9 (FIRE–Cas9), combines these approaches... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....Loures, L. et al. A modular cloning toolkit for genome editing in plants. BMC Plant Biol 20, 179 (2020... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m.... can be used to integrate the tagged AID* into genomic sites of interests via CRISPR techniques. This ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....on the Addgene Blog Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about...