We narrowed to 406 results for: mag

1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...outshines FRET — BiFC experiments only require that you image and measure the fluorescence of a single FP. Your...

2. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ...more immediate and dire than Zimmer could have imagined. Does this detract from the quality of the book...

3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ...rolling mean gave us a crude insight into the magnitude of the effect COVID-19 had on our data. Because...

4. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...work for all bacteria; may cause plasmid/host DNA damage. Choosing an antibiotic resistance Choosing the...

5. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ...).        Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015 under CC-BY ...

6. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...applicable to those positions you have already held. Imagine this situation: You’ve spent hours scouring a company...

7. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ... in a single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has,...

8. CRISPR Antimicrobials

   Type

   Blog Post

   Published

   May 3, 2016, 2:30 p.m.

   ... resensitization, or may also cause cell death. Image from Beisel et al., licensed under a Creative Commons...

9. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...within a plate that can hold up to 96 tubes (see image below). Each plate has numbers and letters to give...

10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...hundreds of plasmid requests out each day. As you may imagine, it keeps all of us quite busy. Though there still...

11. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses...

12. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...popular Chinese social media platforms.  As you can imagine, shipping to so many countries means that our shipping...

13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    .... Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish...

14. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...

15. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... Host killing (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a...

16. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ...transcription factor binding sites) As you might imagine, it’s not easy for humans to balance all of these...

17. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution...

18. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...enthusiasts to start a microscopy seminar to connect the imaging community and to create a platform for discussions...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune...

20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ... Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal of Neuroscience...