We narrowed to 403 results for: mag

1. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...work for all bacteria; may cause plasmid/host DNA damage. Choosing an antibiotic resistance Choosing the...

2. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ...).        Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015 under CC-BY ...

3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed PMID: 25307933...

4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ... in a single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has,...

5. CRISPR Antimicrobials

   Type

   Blog Post

   Published

   May 3, 2016, 2:30 p.m.

   ... resensitization, or may also cause cell death. Image from Beisel et al., licensed under a Creative Commons...

6. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...within a plate that can hold up to 96 tubes (see image below). Each plate has numbers and letters to give...

7. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   ...hundreds of plasmid requests out each day. As you may imagine, it keeps all of us quite busy. Though there still...

8. Plasmids 101: Protein tags

   Type

   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses...

9. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution...

10. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...enthusiasts to start a microscopy seminar to connect the imaging community and to create a platform for discussions...

11. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...Many thanks to Eric Perkins and Stephanie Hays for images used in this post. Klaus Wanisch is an Outreach...

12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune...

13. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...PMID: 9618448 Diaz R, Rech J, Bouet J-Y (2015) Imaging centromere-based incompatibilities: Insights into...

14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...Singleton. Figure 1: The Binning Singletons logo. Image: James et al., 2020. Help me stranger - Finding...

15. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    .... Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish...

16. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...

17. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...coat! These are the things the rest of the world imagines when they think “scientist.” But leaving the bench...

18. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ... Scholar at the Pennsylvania State University. Imagine you are dealing with a defective gene, Xm, the ...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... Host killing (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a...

20. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    Published

    Nov. 10, 2016, 3:30 p.m.

    ...the word ‘antibiotic’, very often we immediately imagine a prescription for a bacterial infection. While...