Skip to main content

We narrowed to 689 results for: mos

Showing: 341 - 360 of 689 results
  1. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...s writing will capture the attention of even the most skeptical reader of historical fiction (I can personally...’s The True Deceiver. There have been books that most of the group really disliked, some that everyone...
  2. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...had to think creatively so as not to develop the most obvious strategy which  also could have been proposed... In the end, the students had fun while applying most of the concepts required by the course and some ...
  3. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...bench scientist who began working remotely recently? Most of us at Addgene began working from home last week... negative data or publish brief, novel findings. Most Micropublications contain one figure. If you aren...
  4. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...Yamanaka himself, during his keynote address, appeared almost incredulous that the field has exploded so quickly...applications with the potential to save lives. While most scientists recognize and appreciate the importance...
  5. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether endocytosis,...cartoony explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis,...
  6. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...meeting with no idea why you are there. Make the most of everyone’s time by considering the level of meeting...to meet monthly, but we found our meetings took almost two hours and we felt out of touch. By moving to...
  7. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...Updated Mar 26, 2020. At their most basic level, CRISPR/Cas9 genome editing systems use a non-specific...crRNAs from pre-crRNAs derived from CRISPR arrays. In most systems used for CRISPR-mediated genome editing,...
  8. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...pathways with sophisticated gene expression controls. Most cell types do not respond to low-intensity light...number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter ...
  9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...fluorescent proteins in common use (particularly not the most recently published ones), they can be a good starting...proteins the green and red proteins are generally the most detectable and so should be used to tag your least...
  10. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...goals. These first hires will set the tone and atmosphere that future hires will look up to." She also ... "Running Your Lab" booklet. What has been the most surprising thing about becoming a PI? Tom Ellis ...
  11. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...educational resources. Filters We identified the most useful kinds of information per resource category... details that make our new search engine unique. Most importantly, with this new implementation, we laid...
  12. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    .... While some developers had science backgrounds, most did not. It was my job to translate complex lab ...front-end and back-end coding frameworks to use. I mostly sit and listen to stay informed on the details,...
  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...available to the research community.    Validation For most cloning projects, the next step would be to validate...digest of the PCR products prior to cloning, which in most cases left the functional light chain intact, the...
  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Overall, these approaches have two main drawbacks: 1) Most rely on transfection of more than one vector to ...lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments, a single vector ...
  15. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...promoters Chemically regulated promoters are among the most common inducible promoters. The positive inducible... agents to allow promoter activation. One of the most commonly used prokaryotic promoters is the negative...
Showing: 341 - 360 of 689 results