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We narrowed to 662 results for: mpo

Showing: 341 - 360 of 662 results

  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...

  2. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...control of kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic ...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity Empty rAAV has a cargo...take the next step – selecting your AAV and AAV components (serotype and promoter) for use in your experiment...

  4. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...Postdoc Wages Read about the Advocating for Science Symposium and Workshop Learn about a Variety of Science ...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...considerations of the laboratory make object composition a smarter choice than inheritance. Some containers...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...inverse orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

  8. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...that the body’s architecture and plumbing are important. We started with learning the head is connected...B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult...

  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...interest. However, FACS is unable to spatially and temporally target single cells. Now, Francois St-Pierre’...way to isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures...

  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...polyclonal antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...

  14. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...editing application. And optimizing its sequence is important, as prime editing efficiency is affected by the...nucleotides. For longer templates, it is even more important to test different lengths and sequences, as unintended...

  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...directly to buffer without a re-wetting step. Important: do not dry your membrane with antibodies on it...ThermoFisher, or Millipore Sigma. We’ll share the basic composition of these solutions below. Alternatively, companies...

  17. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...fragment, any additional recombination events are impossible even in the presence of Cre recombinase. This...in the absence of the endogenous gene. Spatio-temporal expression of optogenetic reporters: Since most...

  18. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...expression levels, the terminator also plays an important role in RNA processing and contributes to variability...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...quantified using qPCR primers targeting specific viral components such as LTRs, gag, WPRE, antibiotic resistance-genes...genomic DNA isolated, and primers targetting viral components or the transgene itself are used to quantify ...

  20. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too...you won't get as much out of it and it will be impossible to schedule. In the pre-event interviews, the...
Showing: 341 - 360 of 662 results