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We narrowed to 745 results for: nes

Showing: 341 - 360 of 745 results

  1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...to interfere with the function of resident ER chaperones and may impact cellular function or possibly ... blue or cyan and yellow moxFPs. The inherent brightness and spectral characteristics of the moxFPs are...negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using CRISPR ...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...demonstrate a POI’s absence. It also helps confirm that lanes are loaded evenly. Created with BioRender.com. ...blot. So how can you accurately compare different lanes? In relative quantification, the POI bands are normalized...blue) to infer or calculate things about earlier ones. In practice, multiple sources of error (red) influence...detection range of the assay is between the two dotted lines. Created with BioRender.com. To see why this...

  3. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...powerful site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite... chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination... Increased Efficiency of CRISPR/Cas-mediated Transgenesis (2018). Pubmed. The Road Less Traveled: Strategies... Increased Efficiency of CRISPR/Cas-mediated Transgenesis (2018). Pubmed. CRISPR References: Genome ...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...   Some phage display libraries screen antibody genes from B cells of immunized animals, but it's possible...screening scFv generated from the B cell antibody genes from diseased or vaccinated humans. How do you produce...cytoplasm. expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm...also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune, and/or inflammatory...

  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...typical PCR machines, the authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even...approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer...mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips, Agarose gel ...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... intracellularly due to a lower dependency on magnesium and higher thermal stability, making it a potentially...and were presented in the study to support the uniqueness of the photostability of the Corn-DFHO complex...biotin. Two of these variants exhibit increased brightness compared to Mango, and all are photostable and...

  7. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...functions. Experiments come next, testing the correctness of the hypothesis. The results of experiments...organisms (GMOs), the absence of a link between vaccines and autism, and other hypotheses mount yet policies...some non-political issues are split along party lines (18). Feeling like an apple will fall up instead...related to the following subjects: Climate change Vaccines GMOs Transgender issues Reproductive health Immigration...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well as a latent microglial line, treatment ...via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9...Cas9 have also been used to create mutations in genes essential to HIV-1 replication. However, this approach...modification targeting either the LTRs or essential genes. Many of these escape mutations were located near...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse lines to restrict transgene...used and emerging capsids to help you decide which ones may be suitable for your experiment. Like all viral...inducing an immune response against the capsids or transgenes. Sensory neurons and liver appear especially ...appropriate cell type-specific promoters or Cre lines. Spatial transcriptomics suggests that AAV-PHP.eB... Seed, C., & Deverman, B. E. (2019). Delivering genes across the blood-brain barrier: LY6A, a novel cellular...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...decision. If I’d spent my senior year isolating pheromones from various C. elegans mutants by myself, I ...etc. If you find that there is not someone (or someones) taking on these responsibilities on a team to...really starts functioning (or malfunctioning). Clear lines of communication Few people actually like meetings...purpose, an excellent leader, and perfectly clear lines of communication, but if teammates don’t trust each...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... estate mantra, the choice of serotype (which determines which tissues and cells an AAV can infect) can... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):... PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of adeno-associated...

  12. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...methods. However, most investigators use single cell clones for in vitro CRISPR experiments. The likelihood...may not be worth the cost and labor when single clones are selected. Conversely, translational research..., Yanfang, et al. "High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...mutations triggered by engineered nucleases." G3: Genes| Genomes| Genetics 5.3 (2015): 407-415. PubMed PMID...

  13. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... analysis, neighborhood analysis of surrounding genes, and experimental data. In some cases, CRISPR systems...researchers began building optimal plasmids to harness the power of Class 1 systems. Class 1 CRISPR systems...cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for inserting...A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be adapted...

  14. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ..., energy is propagated into the sample via an evanescent wave that only excites fluorophores within a ...through the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes that project...sheets of excitation light through multiple focal planes of a sample may also be useful for providing fast...Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...protein, you can site-specifically label it with finesse using tRNA suppression technology (Fig. 1) (Dumas...cells nonspecifically, and they cannot cross cell membranes, so they are best used either with plasma membrane... Most importantly, these compounds cross cell membranes, thus enabling FRET measurements in intact cells...Fessenden is currently an Assistant Professor in the Anesthesia Research department at Brigham and Women’s Hospital...

  16. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...sequence between two target sites. Modifying multiple genes at once. Using multiplexed gRNAs can target multiple...CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based MAGE (Multiplex...simultaneous multiplex editing of six different genes. This system doesn’t rely on any additional or host-dependent.... Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

  17. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ... experience and judgement to a wide variety of business decisions that are not “legal” at all. Q: How ...Addgene board member? BN: Having seen so much in the business world from the mindset of a lawyer can bring a...if we do this, then what?” I hope to bring that business experience and judgement to the organization and...

  19. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...writing, clinical, regulatory, product development, business development, consulting, policy, big data and ...medicine and health, education, law, accounting, business specialties, theology, and the arts, and requiring...your work has impacted your field (i.e. finding vaccines for deadly disease, building algorithms to program...regulatory, project management, alliance management, business development, finance, and operations. To learn...

  20. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...science and business or programming were new to some, who were surprised at “how much business there is ...Last week, 20 students and four chaperones from Denis-Yarmouth Regional High School visited our labs ...
Showing: 341 - 360 of 745 results