We narrowed to 507 results for: otos
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TypeBlog PostPublishedJune 26, 2014, 2:23 p.m....asking your sequencing facility to apply a special protocol for a difficult template. Anything more and you...
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Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m.... available at Addgene CRISPR-Cas9 References, Protocols, and more References: Generation of Gene-Modified... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....Without A Kit by Leah Schiesow We added to our protocol collection this year and along with it, wrote ... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....base editor can be used for A•T to G•C edits in protoplasts or plants of rice and wheat. Read the paper... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m....to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....Browse Additional Viral Vectors Find Viral Vector Protocols ... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog PostPublishedNov. 27, 2013, 2:54 p.m....cleaning out the freezers to writing new plasmid protocols for our website. Free lunch is provided for participants... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in Mammalian Cell Lines ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....the genomes of S. cerevisiae and S. pombe. The protocol is surprisingly simple, requiring only a PCR product... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....us @Addgene. References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A systematic identification and... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....experiments, and nLightG was successfully trialed in two-photon in vivo experiments. nLight indicators are more... -
Future of Research Conference - Remarkable Opening Session
TypeBlog PostPublishedOct. 7, 2014, 3 p.m....The keynote speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m.... (e.g., apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....might need larger numbers.” Further details and protocols are available online on Addgene's CRISPR Library... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m.... Find AAVs for your experiment Browse our AAV protocols ... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m.... ligand from the Bryan Roth lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....plasmid stocks. The guide also contains multiple protocols and troubleshooting tips to make plasmid usage... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor retains its catalytic... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m.... using CRISPR-associated transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596...