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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 341 - 360 of 628 results

  1. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...   Biological Processes  If you’re feeling relieved that you’re working with native proteins instead...instead of denatured ones - well, don’t relax just yet. Proteins often change shapes and epitope accessibility...

  2. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...coming soon! These vectors listed below should be released at Addgene in the next 6 months, pending quality...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...determine if our tubes were permeable to the CO2 released from dry ice, and whether this affected the pH...tested whether venting the tubes prior to thaw released the CO2 from the headspace, thus mitigating the...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...give researchers the ability to monitor glutamate release from neurons and other brain cells in vivo. Glutamate...differentiating between synaptic vs. extrasynaptic glutamate release. Building on the previous generation of the SF-...

  7. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...Meetings are for talking, not reading: Send around relevant documents, like proposals to review or project...discussions can happen “off-line” with just one or two relevant people. How often do you need to meet? Use trial...

  8. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...process had just been discovered, the mechanism was barely understood.” Doench’s graduate work helped expand...regulatory process. This early research is still relevant to his work at the Broad Institute today, where...

  9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...lecture at iBiology. Commonly used filter sets & relevant fluorescent proteins Commonly used filter sets...

  10. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...bacteria, plasmids used for stable transfections rarely contain an ORI since the integrated DNA will be... Stably transfected cells may be considered an entirely new cell line from that of the original parental...

  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...cannot convince others that it is important and relevant to them.   When you think about it, virtually ... to forget that you have a much bigger job than merely telling your audience about the work you've done...

  13. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...heat shock. In the case of Hsp70, the heat shock releases heat shock factor 1 (HSF-1), which subsequently...cells and typically only in the same or closely related species from which they were derived. Similarly...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection...

  15. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

  16. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...used to indicate them on the map, direction (if relevant), and types. Types listed are standard GenBank...Addgene software development teams for working tirelessly to implement these improvements. Let us know ...

  18. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...researchers in creating and sharing knowledge, related to the types of materials we help distribute and...had the deeper expertise of our antibody team to rely on. High-impact content like the antibody guide ...

  19. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...by the bacteria after transformation. LIC is a reliable cloning method, but it is limited by its sequence...SLIC), eliminated many of LIC’s constraints by relying on the power of homologous recombination. SLIC ...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...polymerase. E4/E1A activate transcription of other relevant viral genes (including the ones listed previously...
Showing: 341 - 360 of 628 results