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Showing: 341 - 360 of 474 results
  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    .... 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling in... into thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique...
  2. Hot Plasmids: May 2026

    Type
    Blog Post
    Published
    May 5, 2026, 1:15 p.m.
    ...Plus, the MBP-Benzonase plasmid is available to industry as well as academic scientists. And in Rieubon... Application of nLightG2 for dual-color fiber photometry in fear conditioning experiments in mice. A) ...
  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken, and...
  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct...
  5. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell...
  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially...
  7. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J. E., & Gomes, A. V., “Stain-Free...control to β-actin for Western blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j....
  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC... a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7. https://doi.org/10.1038/s41557-023-...
  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...
  10. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...
  11. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ...Biotinylated proteins were isolated, identified by mass spectrometry, and classified by source cell based on single-cell...widefield imaging, two-photon microscopy, and fiber photometry. Plus, both designs offer promising templates...
  12. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov &...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology...
  13. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ... blots, ELISAs, immunoprecipitation, and flow cytometry. The better these tools are, the better the reproducibility...cells. This enables direct screening via flow cytometry for improved binding under defined environmental...
  14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ..., label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques...-based Photoactivatable Transcription System. Chemistry & Biology 22:169–174 . https://doi.org/10.1016...
Showing: 341 - 360 of 474 results