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We narrowed to 772 results for: INA

Showing: 181 - 190 of 772 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...others you’ll encounter include monoclonal and recombinant antibodies). By the end, you’ll have a better...column and from the target protein. Pre-adsorption Finally, polyclonal antibodies may be pre-adsorbed to remove...batch variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no...polyclonal antibody, or select a monoclonal or recombinant antibody instead. Otherwise, you might experience...of background noise compared to monoclonal or recombinant antibodies. This is because the polyclonal antibody...antibodies Learn about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org...

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...websites, Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated... make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical School. We want to give a ... really like this award because you don’t get nominated for it, it just reflects how useful the reagents... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ... A blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS...

  3. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...two non-functional fragments: N-terminal BirA* (N-BirA*) and C-terminal BiraA* (C-BirA*). These two fragments...(PPIs). These interactions are often modeled as binary relationships, i.e. protein A interacts with protein...Split-BioID is that it allows for the validation of a binary PPI as well as the identification of additional...complementation assay that can be used to study binary protein interactions. However, FRET generally only...proteins were tagged with. It’s best to test all combinations and pick the conditions that give the strongest...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...general availability of their recombinant antibodies. Since recombinant antibodies are a newer technology...chose monoclonal antibodies (mAbs) and their recombinant forms (rAbs). mAbs and rAbs have higher homogeneity...produce the same results even to a different sample. Finally, as a lab we discuss the results together, explaining...Zhuhao Wu at the Icahn School of Medicine at Mount Sinai.  References and resources References Baker M. ...ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...-specific diagnostics for the Ebola virus. Our original proof-of-concept work was exciting because it ...the spread of the virus by the Aedes mosquito (Messina et al, 2016). We quickly assembled a team of students...Asian-, African- and American-lineages). Strain discrimination is especially important in the current outbreak...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine...self-assembly for applications in health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid...25417167. PubMed Central PMCID: PMC4243060. 4. Messina, Jane P., et al. "Mapping global environmental ...

  6. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...training, going from intermediate to advanced and finally, to expert.   Figure 1. Visual representation...subcutaneous injections take some practice to get the coordination down. Subcutaneous injections are most often...delivered to the central nervous system via cerebral spinal fluid. The injection site is the space in and around...minimally invasive. Restraint: anesthesia Location: spinal cord Gauge: 30 Volume: up to 0.01 ml (10 µl) Skill...what method you’d like to use, remember that the final decision will likely come down to experimental necessity... 26 < 0.05 mL Advanced Intrathecal Anesthesia Spinal cord 30 up to 0.01 mL Expert Intratracheal Anesthesia...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Validated recombinant antibody toolbox for aminoacyl tRNA synthetases By Kate Harten Recombinant antibodies...library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA synthetases Nanocompartments... axonal projections and putative pre-synaptic terminals with one vector. AAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby...Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing better signal to noise for imaging calcium...dynamics in fine structures. We have expanded our recombinase-dependent virus selection to now include Flp-...

  8. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...°C instead of 37 °C and then screen for ITR recombination with a SmaI or XmaI restriction enzyme digest...RecA, a protein that aids in the homologous recombination of duplicated regions like the ITRs. Restriction... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...standard and AAV transfer plasmids. It’s easy to contaminate a workspace with plasmid, not that I would know...adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human gene therapy methods, ...R.A., & Samulski, R.J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and...

  9. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...cells and eliminate them from the body. While effective, the immune system does not always eliminate every...with the goal of being able to discriminately and effectively eliminate cancerous cells from the body..... What is cancer, really? While cancer is extraordinarily complex at the molecular level, at the cellular...commit itself to the apoptotic pathway and be eliminated from the body. In the case of cancer, however...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...stability and yield. If a plasmid is prone to recombination Use a strain optimized for plasmids with repeat...elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform and prep...Stbl3 have been engineered to reduce plasmid recombination, but are also endA+. endA encodes a thermostable...volume of culture used for inoculation if we get inadequate plasmid DNA yields. Why would volume make a difference...recommended range of the filter or you might inadvertently lower the amount of DNA you purify! Additionally...should be determined for each plasmid/strain combination individually, based on determining the cell density...
Showing: 181 - 190 of 772 results