We narrowed to 855 results for: NTS

...the risk of such events, newer lentiviral packaging systems divide the viral elements among multiple plasmids...Replication competence can arise from recombination events during viral production and poses a biosafety risk...carrying the transfer gene. By dividing the viral elements across multiple plasmids, the risk of recombination...for transfection can make these optimization experiments a bit more challenging. With these considerations... thereby increasing the number of recombination events that are required to generate RCL. To overcome ...drive high levels of expression of certain viral components; according the manufacturer these changes result...lentivirus, the plasmids encoding the structural elements, envelope, and transfer gene are transfected into...

...measure these properties, and what fascinating experiments could push these tools even further? All of these...and find further tips for using FRET in your experiments here.     What is this FRET you speak of? Förster... acceptor In order for FRET to occur several constraints must be met. The donor and acceptor fluorophores...improved dynamic range for fluorescence lifetime measurements." PloS one 10.8 (2015): e0134436. PubMed PMID...the probe. More on these biosensors later on). Experiments using intermolecular FRET probes are typically...reference standards can be used to validate FRET measurements and serve as a type of positive control. In ...questions about cellular processes. Currently, FRET experiments can probe protein-protein interactions, measure...

... but these reagents and strategies are not available to those working with yeast or plants. Using MoClo...the basics of what MoClo is and what types of experiments it enables. What is modular cloning? Modular ...assembly. Simply put, it assembles individual DNA components into larger units or circuits with functional...and a ligase are used to assemble multiple DNA fragments together in a one-tube reaction. Even though most... test a variety of different orientations and components within the system to maximize production potential...multiplexed gRNA systems as well as additional components such as selectable markers, promoters, enhancers...is especially useful for more complex CRISPR experiments (e.g., many guides/parts delivered) in certain...

... Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI.     ...COVID-19 research in other labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends...cellular receptor. To more easily study the spike variants, scientists have developed ways to pseudotype ...lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-... has had many opportunities to evolve. Several variants are now circulating and plasmids containing these...pseudovirus and those encoding different spike protein variants that can be incorporated into pseudoviruses. The...at neutralizing the different SARS-CoV-2 spike variants. You can learn more about their research in this...

...decisions. In my work as a thesis coach for graduate students, I heard about supervisors in all of the above...also considering the needs of other people. Some students and postdoctoral fellows are hesitant about expressing...help you to become more assertive and resolve disagreements with your supervisor, even if he or she is a...will meet both of your needs. 6) Put important agreements in writing One of the most frequent sources ...approximate timelines. Be sure that you keep your commitments, and if for some reason you are not able to, ...they can count on you to follow through on your commitments. Assertiveness is a skill that will help you ...supervisors might try to intimidate you with negative comments (hostile-aggressives, complainers, know-it all...

...technologies have transformed the palette of experiments possible in a wide range of organisms and cell.... elegans approaches rapidly enrich for editing events without the need for any selective marker to remain...the awesome power of genetic selection. Knock-in events tend to be rare, and the challenge with any experiment...in any genetic background, but require variable amounts of experimental manipulation and screening.  A ...screening 768 F1 animals). Recovering rare editing events in a sea of unedited animals struck me as a “needle...into the homologous recombination repair pathway. Reagents required to perform pha-1 co-conversion are available...knock-ins (Ghanta et al., 2020). Together, these improvements from the Mello lab resulted in strikingly high...

...education? Let us know about your educational events in the comments section below! Additional Resources Visit...Last week, 20 students and four chaperones from Denis-Yarmouth Regional High School visited our labs ...Learning about careers at Addgene Afterwards, the students split into two groups for an in-depth look at ...Biology Lianna Swanson was very popular, with the students excited to see a real working lab full of busy...Operations Coordinator: “Kory was awesome!”. The students also got to learn about intellectual property ... technology was a rousing success! Many of the students were interested to see so many different career...such a great day, we look forward to hosting more students next year! Does your lab or organization do work...

...majority of the respondents (70%) used a lipofectamine-based method to deliver NgAgo ingredients into the cell... questions about the NgAgo protocols used by respondents (transfection method, amount of each component...successful indel formation at a target site. Also, 41 respondents attempted to use the technique for epitope tagging...Survey The take-home message While most of the respondents have been struggling to achieve genome editing...generation and epitope knockin was achieved by a few respondents suggesting that NgAgo can indeed be optimized... take-home message thus far, based on survey respondents "Final Verdict", is that more time is required...Addgene Blog - Leave suggestions and tips in the comments section below or contact [email protected] if you'd...

...Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...Use a strain optimized for plasmids with repeat elements, such as viral plasmids with inverted terminal... and prep high quality DNA that contains these elements. Be aware of endA+ strains Strains like Stbl3 ...including HB101 and derivatives, release large amounts of carbohydrates that can inhibit the cell lysis...of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression strain...culture media Changing to a richer media or adding supplements can improve your cell density, leading to an ...normally recommended antibiotic concentration. Add supplements to the media You can achieve higher cell density...

...phenotype can lead to a wealth of additional experiments investigating the role of individual genes or...carry out so-called “forward genetic screening experiments”, it is certainly the most robust. In this blog... genome-wide screens and highlight some of the reagents that have been made publicly available through... and versatility. CRISPR/Cas9 consists of two components: the non-specific endonuclease (Cas9) and a single...72005145@N00/5600978712/ What CRISPR-based reagents are available for genome-wide screens? The goal...gRNA-containing plasmids. CRISPR genome wide-screening experiments commonly use lentivirus to deliver a pooled population... mind that libraries are only as good as the experiments you use them for! A well-developed biological...