We narrowed to 525 results for: ARL
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TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....researchers doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility...
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How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....final check before publication. Do research! Nearly everything we write entails a primary literature... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....both a screen and a selection, it is important to clearly define what type of factor you are looking for ... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....Would you recommend any liver specific capsid, particularly one that can target the epithelial cells in ... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....to specific cell types of interest. This is a particularly nice feature as organ-specific injections, or... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....these vectors can exhibit spurious expression, particularly at the injection site (Fischer, KB. 2019). The... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....Scientists have developed calcium indicators spanning nearly the entire visible spectrum (Figure 1). The GCaMP... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....for your experiment Monoclonal antibodies are particularly useful when you want to ensure that the antibody... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....Addgene blog Check out other tools and resources for early career researchers Applying for jobs? Check out ... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m.... make the virus for you. Neuroscientists are particularly interested in using AAVs because they provide... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....most abundant molecules in a cell, this system is clearly also robust enough to ensure high levels of gRNA... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....test was the perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....challenge associated with molecular plant parts, particularly for applied projects intended for commercialization... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....knock-out mice, but for various reasons it is not nearly as efficient in human stem cells. What genome editing... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences in gene expression... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....proteasome machinery to degrade any protein. We are particularly excited about the dTAG system as we can use ...