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Showing: 1 - 20 of 600 results

  2. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...Finding research papers is not particularly hard. There are millions of them. The real challenge is finding... relevant papers. The latest installment of the Early Career Researcher Toolbox will highlight four tools...will become the default PubMed search option in early 2020 and eventually replace the existing version...Learn why scientists should be on Twitter Read our Early Career Research Toolbox blog posts on free tools...

  3. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...paragraphs with many hard-to-read sentences.  Grammarly Grammarly is a lot like spell check, but since it’s...Docs, social media, etc.). The free version of Grammarly also offers basic writing suggestions, like alternative...organizing papers, check out this related blog post: Early Career Researcher Toolbox: Finding Relevant Papers...

  5. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...We’re excited to welcome Karen Katz, Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors... through my work on the audit committee. I’m particularly curious to see how that progresses in 2019 and...BioBuilder and Brookline Interactive. Learn more about Carl Paratore, MBA, CPA Mr. Paratore serves as the General...governance processes. Prior to Tufts Health Plan, Carl worked at both MetLife & Ernst & Young, LLP, auditing...Information Systems Audit and Control Association. Lastly, Carl also serves on the Board of Advisors and Audit Committee...

  7. Optogenetics AAV Preps

    Type
    Collection
    ...red-shifted) mScarlet Constitutive 8 Karl Deisseroth 130998 pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine...red-shifted) mScarlet Constitutive 8 Karl Deisseroth 137159 pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine...red-shifted) oScarlet Cre and Flp dependent 8 Karl Deisseroth 137160 pAAV-nEF-Coff/Fon-ChRmine-oScarlet nEF ChRmine...red-shifted) oScarlet Flp dependent 8 Karl Deisseroth 137161 pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet nEF ChRmine...dependent 8 Karl Deisseroth 125712 pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE CaMKII eOPN3 mScarlet Constitutive...dependent 8 Karl Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA EYFP Cre dependent 1, 5, 9 Karl Deisseroth...

  8. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26966P 26966-AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26969P 26969-AAV5 pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26973P 26973-AAV5 pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...85054 pLifeAct_mScarlet_N1 Actin filaments LifeAct mScarlet Dorus Gadella 85056 pLifeAct_mScarlet-i_N1 Actin...Lin 189770 pEB3-mScarlet3_N1 Microtubules EB3 mScarlet3 Dorus Gadella 189768 pmScarlet3-alphaTubulin_C1...mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I Nucleus 3xNLS mScarlet-I Dorus Gadella 98876 4xmts-mNeonGreen... 98818 4xmts-mScarlet-I Mitochondria 4x Mitochondrial targeting sequence (COX8) mScarlet-I Dorus Gadella...mTurquoise2 Dorus Gadella 85053 pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Dorus Gadella 18982 pHIV-H2BmRFP...mRuby3 Michael Lin 224436 pCS2FA-H2B-mScarlet3 Chromatin H2B mScarlet3 Dorus Gadella 136571 pH2B-emiRFP670...Gadella 98821 Lck-mScarlet-I Plasma membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella ...

  10. Control AAV Preps

    Type
    Collection
    ...pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet...dependent 8 Karl Deisseroth 137136 pAAV-Ef1a-Con/Fon-oScarlet EF1a oScarlet Cre and Flp dependent 8 Karl Deisseroth...Dimidschstein 137135 pAAV-Ef1a-oScarlet EF1a oScarlet Constitutive 8 Karl Deisseroth 174112 pAAV-EF1a-mitoGFP-DIO...Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457 pAAV-hSyn-...137137 pAAV-Ef1a-Con/Foff 2.0-oScarlet EF1a oScarlet Cre dependent 8 Karl Deisseroth 137162 pAAV-Ef1a-Con...Deisseroth 137138 pAAV-Ef1a-Coff/Fon-oScarlet EF1a oScarlet Flp dependent 8 Karl Deisseroth 55650 pAAV-hSyn Con...PHP.eB Karl Deisseroth 114472 pAAV-hSyn-mCherry hSyn mCherry Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth...

  11. Arf GTPase Family

    Type
    Collection
    ... Gateway GTPase Arl4c 10123 201/192 Gateway GTPase Arl4d 379 201 Gateway GTPase Arl5a 26225 179 Bacterial...Gateway GTPase Arl5b 221079 179 Bacterial (pET), Gateway GTPase Arl5c 390790 179 GTPase Arl6 84100 186 Bacterial...Gateway GTPase Arl8a 127829 186 Bacterial (pET), Gateway GTPase Arl8b 55207 186 Gateway GTPase Arl9 132946 187...Gateway GTPase Arl13a 392509 256 GTPase Arl13b 200894 428 Mammalian (pcDNA3) GTPase Arl14 80117 192 Bacterial... Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway GTPase Arl2 402 184 Bacterial... Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl4a 10124 200 Bacterial...187 Bacterial (pET) GTPase Arl10 285598 276; 244 Bacterial (pET) GTPase Arl11 115761 196 Bacterial (pET...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...mCherry Control Karl Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Karl Deisseroth 50459...Cre-dependent Control Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Karl Deisseroth ...Control Karl Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Karl Deisseroth... Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth 55637...Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Karl Deisseroth 87306...Optogenetics Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a Activator Optogenetics Karl Deisseroth...

  13. Biosensor AAV Preps

    Type
    Collection
    ...5, 8 Karl Deisseroth 137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F EF1a GCaMP6f none Cre dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M EF1a GCaMP6m none Cre dependent 8 Karl Deisseroth...Constitutive 8 Karl Deisseroth 137126 pAAV-Ef1a-Con/Fon-sRGECO Ef1a sRGECO none Cre and Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137128 pAAV-Ef1a-Coff/Fon-sRGECO Ef1a sRGECO none Flp dependent 8 Karl Deisseroth...pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Karl Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-...6m-p2A-ChRmine-Kv2.1-WPRE Syn GCaMP6m none Constitutive 8 Karl Deisseroth 137119 pAAV-EF1a-Con/Fon-GCaMP6M EF1a...pAAV-Ef1a-Coff/Fon-GCaMP6M EF1a GCaMP6m none Flp dependent 8 Karl Deisseroth 137165 pAAV-Ef1a-Con/Fon/Von GCaMP 6m...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Cre Yes 137135 pAAV-Ef1a-oScarlet None Yes 137136 pAAV-Ef1a-Con/Fon-oScarlet Cre AND Flp Yes 137137 pAAV-Ef1a-Con...pAAV-Ef1a-Con/Foff 2.0-oScarlet Cre AND NOT Flp FRT/F5 Yes 137138 pAAV-Ef1a-Coff/Fon-oScarlet Flp AND NOT Cre...137158 pAAV-nEF-ChRmine-mScarlet None Yes 137159 pAAV-nEF-Con/Fon-ChRmine-oScarlet Cre AND Flp Yes 137160...Fon-ChRmine-oScarlet Cre AND NOT Flp FRT/F5 Yes 137161 pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet Flp AND NOT..., Awatramani R. 2018. Mapping projections of molecularly defined dopamine neuron subtypes using intersectional...LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A hypothalamus-habenula circuit...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mScarlet 569 594 71 5.3 2.9 h Monomer pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning...Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 h Monomer pmScarlet-H_C1 - Mammalian Expression...mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...pWXC078 - Worm Expression mScarlet3 569 592 78 4.5 37 min Monomer pmScarlet3_C1 - Mammalian Expression pWXC077...Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min Monomer pmScarlet-i_C1 - Mammalian Expression...

  16. Recombinases AAV Preps

    Type
    Collection
    ... a fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre... fusion tag) 1, rg* Karl Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Karl Deisseroth 87306 AAV...Viral Tools 183535 pAAV-CaMKIIa-Flpo CaMKIIa none 8 Karl Deisseroth 236288 pAAV.TBG.PI.FLPo.rBG TBG none ...Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a none 8, rg* Karl Deisseroth *AAVrg = retrograde serotype, produced...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...118744 pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ...pENTR221-VPS35-mScarlet VPS35 mScarlet Parkinson's Johanna Ivaska 215474 pEF.DEST51-VPS35-mScarlet VPS35 mScarlet...Parkinsonism, early-onset Roger Reeves 53822 SYNJ1-170_pCSdest SYNJ1 CMV, SP6 Parkinsonism, early-onset Roger...telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173759...Arrowsmith 184825 pSN837 KIF5A mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827...184827 pSN526 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko...Parkinsonism, early-onset Alessandro Ori 221523 SYNJ1_BirA_Nterm SYNJ1 BirA, Flag CMV Parkinsonism, early-onset...

  18. Fluorescent Proteins: FRET

    Type
    Collection
    ...10 SYFP2 mScarlet3 515 0.68 592 104,000 0.75 6.3 4.8 pSYFP2-C1 , pmScarlet3_C1 , pDx_mScarlet3-SYFP2 mVenus...pLSSmOrange-mKate2 LSSmOrange* mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3_C1 mT-Sapphire* ...and turquoise FRET pairs Gadella et al. (2023) mScarlet3: a brilliant and fast-maturing red fluorescent...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...6R] Mad3 Human Mouse IgG2a 140076 Anti-Arl13b [N295B/66R] Arl13b? Mouse Mouse IgG2a 140077 Anti-ZNF423 ...TrpV3 Rat Mouse IgG2a 188211 Anti-BBS3/Arl6 [N302/10R] BBS3/Arl6 Human Mouse IgG2a 188212 Anti-PSD-95 [... channel Rat Mouse IgG2b 199413 Anti-Arl13b [N295B/66R-2b] Arl13b Rat Mouse IgG2b 199414 Anti-GluN1/NR1...41R] EAAC1 Rat Mouse IgG2a 206616 Anti-Arl13b [N295B/54R] Arl13b Mouse Mouse IgG2a 206618 Anti-GluN3A/NR3A...1] VGlut2 Rat Mouse IgG1 206708 Anti-Arl13b [N295B/66R-1] Arl13b Mouse Mouse IgG1 206709 Anti-NR1 glutamate...rat IgG2a 227041 Arl13b (Mus musculus) recombinant mouse monoclonal antibody. Arl13b Mouse Chimera: M.... N29/29 VGlut2 Rat Mouse 182086 Arl13b scFv [N295B/66] N295B/66 Arl13b Mouse Mouse 182088 GABA(A)R, gamma2...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase...Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal...need for an illumination source. BRET can be particularly useful for in vivo imaging or creating biosensors... useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc (ATP-independent luciferase...luciferase derived from teLuc) fused to mScarlet-I. Find more NanoLuc® tools, including constructs for NanoBRET...
Showing: 1 - 20 of 600 results