...requirements. To solve this problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo...efficiency. Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

...Although plasmids started as a somewhat niche area of research, they are now seen as an ubiquitous tool that ...little bit easier for scientists to conduct their research! Since their discovery in the 1950s, plasmids ...have greatly impacted many areas of biological research and have been key in advancing our knowledge in...

... red light instead of blue, which will allow researchers to independently control the activity of two .... Understanding the brain through optogenetics research Addgene: What are some of the big problems that...Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab Sign ...

...), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles have some advantages...isolation protocol by Maguire and colleagues, other researchers have tried to modify it to improve yields. For... viral protocols and videos Find AAVs for your research ...

... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types of biological...enough to engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly...environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department of...

...the University of Alberta. If you’re like many researchers these days, you are ready to take (if you haven...are often impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease”...knockout of HIV co-receptor CCR5. Nucleic Acids Research 43, 5560–5571 (2015). PubMed PMID: 25964300. PubMed...

...base editors for your research Read our CRISPR guide Find plasmids for your research ...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a...

...expression of recombinant proteins for industrial or research purposes requires stable host cells (the simplest...commercial operators, government agencies, active researchers, as well as fellow iGEM teams. We were the proud...more productive and collaborative environment for research.  We’ve also involved ourselves in extensive outreach...

...-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing...https://doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing...resource on the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies...

...γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus... 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or drive high...Cycle Popular Retroviral Vectors and Their Use in Research More resources on addgene.org Addgene Protocols...

... for your experiment. Research applications Gene expression control Researchers are always wanting to ...fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...

...GPCRs. This collection, called TRUPATH, allows researchers to identify specific G proteins activated by ...s still a lot to learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic...chemogenetics guide Find chemogenetics plasmids for your research ...

...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular biology and...inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM can help...Perfect Plasmid Backbone Find Plasmids for Your Research ...

...engineer and scientist, I like to envision that my research will have a direct impact on healthcare and the...result, I have occasionally pondered translating my research into a startup company. However, like many like-minded...incorporated into Novophage Therapeutics, Inc. in March 2009 and tested in the “market” by competing in ...

... podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what... Yeast Transcription Factors Podcast Segment Researchers from the El-Samad lab recently deposited plasmids...that this level of control will enable future researchers to better simulate physiological gene expression...

...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...low resolution and can’t be used in vivo. Some researchers have used fluorescently tagged DNA-binding proteins...CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single genomic locus,...

...of the different steps in RNA extraction. Researchers at the NIH in the mid 1980’s set out to develop...with chloroform as in the above no-kit method.  Researchers have also developed ways to “stabilize” RNA within...gene expression. There are several things that researchers can do to combat this issue. First and foremost...

...Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...this process is not fully understood, a team of researchers lead by Takayuki Kogure (Kogure et al., 2011)...

...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...editing efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor,...