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We narrowed to 662 results for: CLU

Showing: 361 - 380 of 662 results
  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...
  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have been reported...al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated purification of a specific genomic...
  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...the primer sequences. Multiple plasmids can be included in a single PCR, for example for subcloning genes...vector. The new codon and homologous region can be included at the 5’ end of either primer. Subcloning - To...
  4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...and its RNA directed binding site. These methods include the fusion of an activation domain such as VP16... or tracrRNA sequences can also be modified to include RNA domains that recruit additional transcription...
  5. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...their production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a ...IgG subclasses, so that secondary antibodies exclusively recognize only one primary antibody. This process...
  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...in the detection of microbes. Some applications include measuring viable probiotics and detecting circulating...
  7. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, ...combinations, though frequently those combinations still include at least one element from the original TAP tag....
  8. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ... CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system...sight in that Addgene takes care of everything including MTA and shipping. Addgene realizes the One-Stop...
  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...method can be used for an array of applications including clinical diagnostics (e.g. pathogen or virus detection...Cas12a is initiated. Surrounding trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded...
  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...target the ubiquitin E3 ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein...mScarlet-I3 that were codon-optimized for C. elegans and included artificial introns to improve expression. When...
  11. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...show your enthusiasm for getting the position and include a brief summary of why you are a good applicant...taught for five years at [name of the university]” Include keywords from the job description to help the hiring...
  12. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... in the linings of certain areas of the body, including the lung, the heart, and the abdomen. Long story...primary conventional treatments for mesothelioma include surgery to remove cancerous masses (tumor resection...
  13. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...to the total number of viral capsids (which can include empty capsids that are devoid of a viral genome... et al., 2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which...
  14. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... works on our site. For Addgenies, development includes everything from tracking plasmids from the moment...then go back to this area later and tidy it up. Conclusion While none of us are life science majors, we ...
  15. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...antibiotic resistance develop? Microbes (bacteria included) are very skilled at the delicate art of survival... contributing to the development of resistance include (Figure 1): Over-prescription of antibiotics (...
  16. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...citing research from outside of their disciplines. Included in the article is an interactive chart where you...is of interest. In his research, Scott Spicer concludes that “… the emergence of the video abstract genre...
  17. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...number of potentially hostile posters (this advice included leaving out the term “implicit bias” which is ...rewriting or coming up with answers, I was able to include links to these posts and eBooks. I also have ...
  18. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...burden from getting overwhelming. These mechanisms include efforts like flexible staffing on case teams, where...offices, classes at business schools, and consulting clubs are especially helpful since they both provide up-to-date...
  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...easy to follow seminar See item 2. Attendees will include grad students (that are solicited for feedback)...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...
Showing: 361 - 380 of 662 results