...specific compounds, links to publication references, and cross-references with cell line catalogs and collections...be adjusted based on query, reference, and shared allele search preferences to help you identify proximal...to learn more, check out the Cellosaurus FAQ! References Lorsch JR, Collins FS, Lippincott-Schwartz J....

... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...SpCas9 or GeoCas9 RNPs were incubated in reaction buffer at temperatures ranging from 25-80 °C. Activity...incubated in human plasma diluted with reaction buffer for 8 hours at either 37 °C or 4 °C. Substrate ...gel. SpCas9 is stable when incubated in reaction buffer alone, but has limited activity when incubated ...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...

...encompasses millions of choices including pharma, tech transfer, management consulting, science communication,...of today’s postdoctoral training can directly interfere with a seamless jump into industry.” In his article...interact with scientists just like you What is different about academia? Publishing in peer reviewed journals...Constant worry about grants and renewals What is different about non-academia? Scientific publications are...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...

...work demonstrates that different repair template strategies activate different HDR sub-pathways like synthesis-dependent...live updates! My most popular tweet from the conference is shown above. The blog post I mentioned, John...post-CRISPR cleavage When I first looked at the conference agenda, I was surprised to see that it would ...Having had time to digest my notes, since the conference, I think I may have finally grasped just how ...

...due to genetic drift and the use of genes from different microbial strains. It proved to be impractical... least 96% sequence identity when detecting a reference feature. For a coding sequence feature that may... could miss a common feature if the sequence differences exceed the threshold. This issue is addressed...often come in closely related versions that have different properties. To prevent misidentification, we augment...way for SnapGene users to apply features from a reference strain to newly sequenced strains through our ...

...antibodies for tagging purposes. Different antibodies bind different protein sequences called epitopes...infamous frankenstein, was constructed by grafting different parts together. By combining two optimized elements...living cells. To make these scaffolds specific to different targets, the scientists fused them to sequences...to build a more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004...

...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase...

...cell-free transcription/translation systems (also referred to as TX-TL systems) have been gaining popularity...initial system by using a stronger promoter and different biochemical secondary energy sources to increase...XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry is used to sort populations...XMAS-TREE-based methods at various genomic loci in four different categories.     Brookhouser, N., et al. BMC...10.1186/s12915-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added...

...Khammash lab can also be found here.   References and Resources References Romano E, Baumschlager A, Akmeriç...functional BLADE variants, too. The team tested different linkers between VVD and the DNA-binding domain...the Molecular Biology Reference Download Addgene's Plasmids 101 eBook ... Jennifer Tsang...

...transductions. If you’ve ever wondered how they are different from or similar to the other Ts, we have your ...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...controlled lab usage. Using viral transductions can offer benefits such as tissue-specificity, high efficiency...that you can swap out your virus type to achieve different biological outcomes. For example, some viruses...target cells or organism, some primary cells and differentiated cell types are picky when it comes to transfection...

...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ... and trends “Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network...particular field of study Assess your interest in a different field Outreach activities (e.g. Volunteer...Leadership Networking University technology transfer office positions   Market analyses Patent...Marketing Communication Business models Conference or club organizer Management Leadership...

...numerous cell lines can be generated, each with a different tagged protein of interest, and a universal all-purpose.... Current methods of epitope tagging, however, suffer from many pitfalls: they require construction of... achieve 5-30% tag knock-in efficiency across different stem cell types. This has facilitated easy generation...format and tested parallel tag knock-in for 96 different genes in a single go (Figure 3). We were able ...optimization includes selection of transfection buffer and electroporation pulse program to achieve high...seen daydreaming about sunny days in the UK.   References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso...

... that affect Th2 differentiation and activation, using expression of differentiation factors as the screen...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter... imaging can be performed in intact animals and offers many advantages over traditional measurements such...Gottschalk group hypothesizes that this may be due to different lipid compositions of C. elegans membranes, which...

...heart disease, and type 2 diabetes, across four different types of human cells, including T cells and hematopoietic... interest with only minimal genetic payloads. Different from existing multiplex array architectures, including...when repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be... lab of Xue Sherry Gao at Rice University.    References and Resources More resources on the Addgene blog...Adding Precision and Flexibility to CRISPR Editing References Yuan Q, Gao X (2022) Multiplex base- and prime-editing...

...seeing adoption of the technology in different labs for different applications.” Li’s team noted that ...cells of patients with cystic fibrosis (8).   The difference with CRISPR/Cas9 in comparison to other genome...” said the University of California Berkeley’s Jennifer Doudna. “They are the first direct demonstration...possible through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” ...

...patterns of different AAV serotypes. To help make this easier for scientists, we now offer trial size ...excited to offer these useful tools to the community. Among other new biosensor tools, we now offer the jGCaMP7c...control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent...

...identifiers (PIDs), a long-lasting way to identify and reference documents, files, or physical reagents. What ...and exist online indefinitely. PIDs historically referred to objects that exist online, but this approach...you, we’ll go through examples to highlight the difference between them using a plasmid available through...the lenti-Cas9-VQR plasmid page. Despite their differences, both of these PID formats for lenti-Cas9-VQR...managed by Addgene. How to cite options using the different PID formats The instructions for how to cite any...

...for creative interaction During Zoom meetings, different tools can be used to promote creative interaction...as a picture. I believe that showing something different from time to time breaks up the monologue a little...papers will be written by several authors from different institutes, disciplines, and cultural backgrounds...and only general chatter will be allowed with a different host each time that moderates conversations. This...communities. You can follow him on twitter @salmon_phd References Frassl MA, Hamilton DP, Denfeld BA, de Eyto E...

...events for one organization or go to the same conferences/meetings over and over to get to know the regulars...people by discussing non-work topics and interests. Offer help when you can, instead of just asking for favors...starfish shape. You can ask me about the different arms of this starfish sometime, but as of yesterday...come across your contacts over the years in many different ways. This leads me to my last tip: It’s usually...Browse other blog posts on networking Attending a conference soon? Get tips here Learn about informational...

... the library are differently sized, smaller plasmids may be amplified preferentially, skewing the composition...some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock...successful amplification? To provide a point of reference, Addgene commonly prepares NGS samples using both...overrepresented plasmids (plasmids that were amplified preferentially). You can also rank the plasmids by abundance...