Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 867 results for: FER

Showing: 361 - 380 of 867 results

  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three colors. The...specificity of these orthologs is key: due to differences in the PAM sequences required by each ortholog...specific for telomeric sequences and showed that different fluorescently labeled dCas9 isoforms are efficiently...target sequence. They were also able to label two different pairs of chromosomes using gRNAs specific to sequences...Addgene are excited to see what’s coming next! References Multicolor CRISPR labeling of chromosomal loci...

  2. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...the efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce...from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding...enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases for ...for use in manipulating animal genomes. Nern A, Pfeiffer BD, Svoboda K, Rubin GM. Proc Natl Acad Sci USA...PubMed. Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity...

  3. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc (one...especially excited to attend this event because Jennifer did her graduate work one floor above mine in ...system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity... science to the non-scientists in the room. He offered the great analogy of comparing the CRISPR/Cas9 ...

  4. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...online on Addgene's CRISPR Library Reference page.    References: Genetic Screens in Human Cells Using...GeCKO library, each library has been enriched for different gene targets. These pooled libraries include enrichment...lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9 can be used ...any sequence in the genome. Tips and tricks Wang offers this tip: “It's quite important, at every step ...

  5. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...at how variable Cas9-expression was across the different cells (Figure 3).   Figure 2: Cas9 expression...publication. Pro-Tip: 1. Use multiple gRNAs to target different regions of your gene. I used existing BRAF gRNA...our website. There are a few reasons to target different regions of the gene with multiple gRNAs, but the...comparing the phenotypes of multiple gRNAs. Targeting different regions of the gene is also useful in case the...that would co-migrate on a gel) instead of two different sized digestion products (which could be easier...your way to making new scientific observations. References 1. Rago C, Vogelstein B, Bunz F. Genetic knockouts...

  6. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...SpCas9 or GeoCas9 RNPs were incubated in reaction buffer at temperatures ranging from 25-80 °C. Activity...incubated in human plasma diluted with reaction buffer for 8 hours at either 37 °C or 4 °C. Substrate ...gel. SpCas9 is stable when incubated in reaction buffer alone, but has limited activity when incubated ...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...tools for your next experiment! References and Resources References Wood, V., et al., Hidden in plain...an endogenous gene. Each trap uses a slightly different approach (described in detail below), but fundamentally...kidneys at a specific developmental stage you could infer that the gene which GFP was monitoring was being... these circumstances, trap methods may be the preferred screening option. Promoter traps Promoter trap...

  8. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...specific compounds, links to publication references, and cross-references with cell line catalogs and collections...be adjusted based on query, reference, and shared allele search preferences to help you identify proximal...to learn more, check out the Cellosaurus FAQ! References Lorsch JR, Collins FS, Lippincott-Schwartz J....

  9. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...antibodies, and more!   Resources and References   References Coligan, J. E., Dunn, B. M., Speicher, ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...Nonetheless, this tag can be purified with several different resin types and is a fairly small tag that is ...  Affinity tags vs. other tags There are many different types of tags, including epitope tags (FLAG, Myc...

  10. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend less...their artificial nature, lab plasmids are commonly referred to as “vectors” or “constructs.” To insert a gene...Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular...plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, ...a plasmid vector, molecular biology tools and references, and how to maintain your plasmid stocks. The...

  11. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... with a six-year-old boy named AJ Gonzales who suffers from Fibrodysplasia ossificans progressiva (AKA...first met AJ and his family, he did not seem much different than my second son of similar age. When he came...therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions...superpower for those running on behalf of rare disease sufferers like AJ. As the Blue Lantern Oath says: "In fearful...running on behalf of AJ Gonzales, a young boy suffering from FOP, raising money for NORD for the past ...

  12. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...work demonstrates that different repair template strategies activate different HDR sub-pathways like synthesis-dependent...live updates! My most popular tweet from the conference is shown above. The blog post I mentioned, John...post-CRISPR cleavage When I first looked at the conference agenda, I was surprised to see that it would ...Having had time to digest my notes, since the conference, I think I may have finally grasped just how ...

  13. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ...antibodies for tagging purposes. Different antibodies bind different protein sequences called epitopes...infamous frankenstein, was constructed by grafting different parts together. By combining two optimized elements...living cells. To make these scaffolds specific to different targets, the scientists fused them to sequences...to build a more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004...

  14. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...encompasses millions of choices including pharma, tech transfer, management consulting, science communication,...of today’s postdoctoral training can directly interfere with a seamless jump into industry.” In his article...interact with scientists just like you What is different about academia? Publishing in peer reviewed journals...Constant worry about grants and renewals What is different about non-academia? Scientific publications are...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...

  15. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase...

  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transductions. If you’ve ever wondered how they are different from or similar to the other Ts, we have your ...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...controlled lab usage. Using viral transductions can offer benefits such as tissue-specificity, high efficiency...that you can swap out your virus type to achieve different biological outcomes. For example, some viruses...target cells or organism, some primary cells and differentiated cell types are picky when it comes to transfection...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...cell-free transcription/translation systems (also referred to as TX-TL systems) have been gaining popularity...initial system by using a stronger promoter and different biochemical secondary energy sources to increase...XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry is used to sort populations...XMAS-TREE-based methods at various genomic loci in four different categories.     Brookhouser, N., et al. BMC...10.1186/s12915-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added...

  18. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...Khammash lab can also be found here.   References and Resources References Romano E, Baumschlager A, Akmeriç...functional BLADE variants, too. The team tested different linkers between VVD and the DNA-binding domain...the Molecular Biology Reference Download Addgene's Plasmids 101 eBook ... Jennifer Tsang...

  19. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...due to genetic drift and the use of genes from different microbial strains. It proved to be impractical... least 96% sequence identity when detecting a reference feature. For a coding sequence feature that may... could miss a common feature if the sequence differences exceed the threshold. This issue is addressed...often come in closely related versions that have different properties. To prevent misidentification, we augment...way for SnapGene users to apply features from a reference strain to newly sequenced strains through our ...

  20. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ... and trends “Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network...particular field of study Assess your interest in a different field Outreach activities (e.g. Volunteer...Leadership Networking University technology transfer office positions   Market analyses Patent...Marketing Communication Business models Conference or club organizer Management Leadership...
Showing: 361 - 380 of 867 results