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We narrowed to 709 results for: LIC

Showing: 361 - 380 of 709 results

  1. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... experimental, biotechnological, and medical applications. Researches can visualize proteins in vivo by...protein you need   Your plans for downstream applications In this blog post we will summarize some of...acids that are useful in different downstream applications. Cell-free expression systems however, are expensive...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...instances. If your poster material is nearing publication, consider uploading your work to a preprint server...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...

  4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt...plasmids, and lack the ability to synthesize diaminopimelic acid (DAP), a component of the cell wall. Once...single-gene knockdown experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model ...

  5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ...with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello ...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

  6. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few..., those who have been lucky with NgAgo should explicitly share tips and protocol details with the scientific... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

  7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since...used so far) or for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma...Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer therapy. Elsevier, pp ...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or... used a solution of benzyl alcohol and methyl salicylate which was later modified by others to produce...Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten. Leipzig: S. Hierzel...

  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...manuscript to get reviewed and edited prior to publication, and this will delay the dissemination of your... makes it easy to annotate protocols, make them public for others to use, and add your own comments. Currently...Freedom: The Expansion of Data Sharing in Research Publications by Jim Woodgett        ...

  11. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...

  12. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...your experiment. Shorter amplicons tend to be better. Shorter amplicons make for shorter PCR programs...Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional ...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this...capture antibody interaction and/or the downstream application. Typically, if the target protein will be detected...and an isotype control antibody. Alternative applications Oftentimes, an IP-appropriate antibody simply...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...pooled library DNA, allow the bacteria to grow and replicate, then purify the DNA.     Figure 2: A pooled...protocol. I also recommend searching PubMed for any publications associated with the library and reading their...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...

  16. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    .... Use in silico cloning tools When available, always perform your MoClo assemblies in silico before you...Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden Gate Cloning ...

  17. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR efficiency...8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL, Tewari M... abundant targets: from variable nonsense to publication quality data." Scientific Reports 7 (2017). PubMed...

  18. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...our loved ones, and become a positive force for public health. Visit Addgene's Microbiology Pages What...Microbes (bacteria included) are very skilled at the delicate art of survival. Existing virtually ubiquitously...about health equity, science communication, and public health.     References 1. O'Neill, J. "Tackling...

  19. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...detrimental as too little, resulting in MECP2 duplication syndrome or Rett Syndrome, respectively. One ...editing, MECP2 reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts....0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY (2013) An AT-Hook Domain in MeCP2...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...
Showing: 361 - 380 of 709 results