We narrowed to 716 results for: Met

... et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision. Our...or double stranded donor DNA with other design parameters. Example single stranded donor DNA and a presumptive...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

...probe and build new systems. Such goals require methods to directly activate and inactivate biological ...that prints a chemical image, as well as a genetic method for the computational problem of edge detection...identified in bacteria, and with similar optimization methods, many of these systems could be made suitable for...

...transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the ... depending on your interests. You can look at something as basic as survival. For those interested in ...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

...both the biological system as well as the imaging method, we recommend basing your selection on a small-...is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the value of EGFP). The higher the...practical brightness takes all application specific parameters into account, including the specs of your microscope...

...protein structure. Sometimes this provides a great deal of clarity. But, often these methods don’t agree. Confusing... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ...

...gene a megabase away, or looking at the histone methylation status at a double-strand break that needs repairing...guest bloggers have written posts describing new methods and tools for studying chromatin and the epigenome... and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA Interactions CRISPRainbow...

...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing ... al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated...

... a look at a few screening methods. Blue-white screen A widely used method of screening is the blue-white...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...

... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a .... And, naturally, rarely are the optimization parameters the same for all three components, meaning that...Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural Amino...

...understand how the brain works. There are two methods of neuronal tracing (Fig. 1): one to visualize ...catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo, 2016). Discrepancies...neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon is...

...variety of cloning techniques and troubleshooting methodologies drew me to learn more about the process that...immunofluorescence-immunocytochemistry (IF-ICC.) This method was chosen because cells transfected with a plasmid...available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for construction and...

...Clouse While many CRISPR-based genetic screening methods have primarily used Cas9 to knock out genes, researchers... screening toolkit by optimizing a Cas12a-based method for screens. Cas12a’s ability to perform multiplex...in parallel. Lastly, this Cas12a-based screening method was used to create a genome-wide library capable...

... can change depending on the location. Current methods for visualizing the location and function of microbes...reporters have limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer...and create ultrasound contrast. At a very low volumetric density and with a resolution of less than 100...

...transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies ... hundreds of transcription factors. We wanted something that would allow automation. This design cuts ...pressure on many other labs that may be doing something similar. There may be other variants of this that...

...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that...models, and software tools in the Materials and methods section of their manuscript to make resources easier...information you provide on data, materials, and methods, the more valuable your paper will be to the scientific...

...to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients...number of genes involved in cardiovascular and metabolic diseases. We are knocking out genes that have ...to examine the effect of a gene on cholesterol metabolism. The TALEN kit is quite useful in generating ...

...pharmacological or genetic approaches, but only a few such methods exist and they only work for a subset of kinases...kinases. The Problem:  There is no common method for creating optically controlled kinases. The Solution:...KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating other signaling pathways...

...efficiency was similar to that of a comparable FISH method. Importantly, they did not observe labeling when...with both intronic and exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene...genome editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed...

...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

...super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...