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We narrowed to 849 results for: NTS

Showing: 361 - 380 of 849 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... in vivo experiments, and nLightG was successfully trialed in two-photon in vivo experiments. nLight indicators... work on further improvements. In particular, they’re focused on generating variants with improved affinity...allows for increased options for multiplexing in experiments, including ones that study alpha2-AR. Selectivity...

  2. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...scale: Look at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer...corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your protein of... to be helping the Davidson lab to share their reagents. "We are proud to be able to bring such an important...work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson, Michelle Baird...

  3. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...Worcester Polytechnic Institute. Though some WPI students chose to go the solo route for their Major Qualifying... the first place. The famous discoveries and experiments I’d been learning about for years were usually...year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy. As it...substantially and have plenty of ideas for further improvements. Types of teams There are, of course, many ...series of tasks with precisely defined goals and endpoints. “Project” is a very broad term since a project...too much work If you are one of those graduate students putting in 60-80 hours a week in the lab, guess...

  4. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...with both evolved and engineered adjustments to the other components. It drove 19% integration in HEK293T...emerging world of gene therapy, highly tailored treatments to specific mutations require individualized ...intervention for a variety of loss-of-function mutants could provide a streamlined, one-size-fits-most...achieve insertion of long sequences. In their experiments, PASSIGE had higher efficiency, but evoCAST required...

  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...Riordan, and Victor Di Rita, and, last, homework assignments. The instructors are leaders in the field of ...publications. My classmates consisted of graduate students, post-grads, and early career scientists. Over... on that week’s topic by the moderators. The assignments, which consisted of reading that class’s section... wordsmith. But first there was an assignment. Students coming to the writing workshop in D.C. had to ...Pseudomonas aeruginosa. Follow him on twitter @100percentSDS.     Additional Resources on the Addgene Blog...

  6. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...digestion, fragments are released from nuclei by a short incubation at 37 °C. These short DNA fragments can ...differential access to transcriptional regulatory elements is controlled during development using the Drosophila... preparation and high-throughput sequencing. Improvements to the CUT&RUN method and using it in your own...Henikoff lab published a preprint paper outlining improvements to the initial CUT&RUN protocol, the most significant...

  7. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... the method is powerful and offers immediate measurements in a live animal that can be correlated to behavior...fluoresces when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit...peaks of DA release.  Using dLight1 sensors in experiments The Tian lab used dLight1 both in brain slices...receptors, respectively). Using GRABDA sensors in experiments The Li lab used GRABDA in drosophila, zebrafish...here! Overall these sensors are powerful new developments in the detection and measurement of neurotransmitters...

  8. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...offer two simple steps to welcome these unforeseen moments. Widening your search The first step towards inviting...breakthroughs often happen when we allow ourselves moments of release and mental freedom. Practical approaches...embraces the unexpected and encourages exploration of tangents or seemingly unrelated ideas. It's beneficial ...fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling in the... contemplate our approach to life and how our viewpoints influence our encounters. It underscores the ...

  9. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS... known to trigger seed germination of parasitic plants, this approach was also used to build and validate...Qi's lab, allows simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified...in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and reduced...sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff...

  10. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...Reading and exploration including replications and experiments resulting in “negative data” often dominate the...For instance, long before a study with multiple patients was published, Zika researcher Christa Osuna collated... interim research products (pre-registration, preprints, data, software, micropublications) in grant proposals...you the hard work of reading papers and doing experiments but we can definitely give you credit for it ...Addgene Blog Use Apps to Save Time and Track Your Experiments Use video to share your science at We Share Science...

  11. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...academia, it is especially important for graduate students and postdocs to get a perspective on other types...use of resources and pay attention to budget constraints Academic science is much like industry but the...science’s sake More opportunity to train and teach students Collaborations may be less “complicated” - for...tell you to pick up the pace Constant worry about grants and renewals What is different about non-academia...apple can change entire culture) Specific requirements for hiring more common Likelier to hire on ...

  12. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color...therefore can be used in immunoprecipitation experiments with other fluorescent proteins. Find mNeonGreen... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and...

  13. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved in ...weeks and with labs worldwide closing for unknown amounts of time, we were facing a financial crisis. As ... resource for scientists that helps them share reagents — leading to open, reproducible science,” said...More scientists are sharing plasmids and other reagents every day, and this crisis has highlighted the...

  14. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ... about Addgene. “The mission to share critical reagents such as plasmids and viral stocks for researchers...otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier, PhD Frank Stegmeier...targets that have the biggest potential to transform patients' lives. Previously Stegmeier was the Head of Oncology...see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...publication that could advance treatments for a rare disease Adult patients or caregivers affected by the... others partner with formal learning to engage students at all levels.      Jargon is field-specific terminology...minorities in your local high school who are also students in AP biology classes Creating a podcast about...explicitly exclude people outside of the original recipients. Instead, it targets a specific audience - giving...

  16. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...able to order ready-to-use antibodies for your experiments directly from Addgene. How Addgene is Reimagining...applications, enabling you to better plan your experiments. To promote reproducibility, Addgene will have...resource may also expand to include other affinity reagents, such as nanobodies and ScFvs, based on community...applications. In addition, basic and advanced affinity reagents topics are discussed on Addgene’s blog and Antibody...recombinant antibodies or other recombinant affinity reagents, share your plasmids via Addgene! Email deposit...

  17. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...They provide accounts of their research struggles in papers. They recount accomplishments in talks and... pay attention. Highlight your lowest points and highest points and make clear what was difficult and ...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster...

  18. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...culture and in live zebrafish. For cell culture experiments, only dL5**-expressing cells treated with MG-...neighboring bystander wild-type cells.   Zebrafish experiments with MG-2I-dL5** were performed with embryos ...Zebrafish are an ideal model organism for these experiments because developing embryos are transparent, so...also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed to YFP and RFP...your experiences using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato...

  19. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...digested with ribonucleases, ribosomes protect the segments of mRNA to which they are bound. These regions...color switching, TRICK requires the following components: a GFP protein that binds to the coding region...the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry labeled mRNA and keeps...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...coding sequence and 3’ UTR. Before beginning experiments, it’s important to verify that these changes ...

  20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...first design is a whole cell biosensor with two components - an enzyme that breaks down the HMO of interest...communication skills throughout her training and wants to make her work understandable to people both inside...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein...
Showing: 361 - 380 of 849 results