We narrowed to 488 results for: RET
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TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...
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Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m.... specific proteins within a system. This can be pretty complicated given that a single cell has tens of... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....Amsterdam) where she develops genetically encoded FRET based biosensors. Additional Resources on the... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping. In the... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....storage vector to the pAWp12 lentiviral vector should retain the diversity in your gRNA library, but this should... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....mutants coding for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m.... Boston 2015 where we hope to begin developing concrete solutions to the problems looming over the academic... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....signaling to peripheral effector organs, which then return information such as nutritional status. Within ... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....digital academic publishing for a year, I’ve since returned to pgEd as a full-time staff member for the past... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....AdV) are devoid of all viral coding sequences and retain only the ITRs and packaging signal in cis. Therefore... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....Setting up a basic protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....conclusions should be clearly identified and interpreted in less than a couple of minutes. How does one...