We narrowed to 459 results for: SHI

1. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

2. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   Published

   April 13, 2023, 1:15 p.m.

   ...-Cas9 tools have been engineered to introduce frameshift mutations in genes of interest, turning expression...

3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...blue light which revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead...

4. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ...consequently about a two-thirds chance of causing a frameshift mutation. Of some importance, the deletion can...

5. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   Published

   July 22, 2014, 5:46 p.m.

   ...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

6. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   Published

   Dec. 10, 2013, 3:13 p.m.

   ...desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the...

7. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   Published

   June 27, 2019, 12:25 p.m.

   ...: 30643213. PubMed Central PMCID: PMC6467691. Takahashi, Melissa K., et al. "A low-cost paper-based synthetic...

8. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   Published

   July 25, 2017, 1:20 p.m.

   ...photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains on either side...

9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ...does not depend on excitation power in a linear fashion. This implies that reducing the power 2-fold does...

11. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory...

13. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

14. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage...

15. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed PMID: 26427452. Issa, ...

16. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...things go wrong, replace them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up...

17. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate protein...

18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...genome engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes...

19. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...

20. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...