We narrowed to 415 results for: genome

1. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...Loures, L. et al. A modular cloning toolkit for genome editing in plants. BMC Plant Biol 20, 179 (2020...

2. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org...

3. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about...

4. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ... Cre. Could Cas9-DOG be developed soon to allow genome modification only in a small subset of cells? Other...

5. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research Associate at the...

6. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   Published

   Oct. 8, 2024, 1:15 p.m.

   ...classifications are still being created as whole-genome sequencing becomes more relevant (Garcillán-Barcia...

7. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...protein identified exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells...

8. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ... types of DNA, such as cDNA, PCR fragments, or genomic DNA. Also remember that commercially available ...

9. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ...expression of genes integrated into the insect cell genome. Both of these types of insect expression systems...

10. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...induction of T7 polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced...

11. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...questions around climate change, biodiversity, or genomes requires lots of data. Get your community involved...

12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...carries a small, approximately 4.7 kb single stranded genome. AAV belongs to the family Parvoviridae and requires...

13. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...sequence (Epigenetics literally means ‘on top of the genome’). Epigenetic modifications can result in phenotypic...

14. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk...

15. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...Model Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids...

16. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ...extremely accurate application, even in areas of the genome that are difficult to accurately read (Bell, 2018...

17. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...been viewed 219,289 times, while Validating Your Genome Edit has 76,011 views.  Has all of this work helped...

18. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...amplifies a product of the expected size, you know the genome of your bacteria already contains your target sequence...

19. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...generated offspring tomato plants that inherited the genomic edit but not the editing machinery. Together, these...

20. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ... that they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr...